| 2091 In-vivo Imaging of Oral Mucositis in an Animal Model | ||
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P. WILDER-SMITH1, M.J. HAMMER-WILSON1, J. ZHANG1, Q. WANG1, K. OSANN1, Z. CHEN1, H. WIGDOR2, J. SCHWARTZ3, and J. EPSTEIN4, 1University of California Irvine, USA, 2College of Dentistry, Chicago, IL, USA, 3Universiity of Illinois @ Chicago, USA, 4University of Illinois - Chicago, USA Objectives: In this feasibility study, the ability of non-invasive Optical Coherence Tomography (OCT) and Optical Doppler Tomography (ODT) to detect and characterize chemotherapy-induced oral mucositis was evaluated. Methods: Cheek pouches of 10 Syrian Golden hamsters were imaged using OCT/ODT during development of chemotherapy-induced mucositis. Intra-peritoneal injections of 5-fluorouracil and mechanical irritation induced oral lesions. At 2, 4, 7, 11 days, 1 hamster was sacrificed and processed for histopathology. OCT images were visually examined; ODT results were semi-quantified. Imaging data were compared with histologic findings. Results: During the development of mucositis, OCT/ODT identified the following events: (1) change in epithelial thickness (beginning on day 2), (2) loss of surface keratinized layer continuity (beginning on day 4), (3) loss of epithelial (day 4 onwards) and submucosal integrity (day 7 onwards), (4) Changes in axial blood flow velocity (increased day 2,4; decreased day 7), (5) changes in blood vessel size (diameter doubled day 2; quadrupled day 4; unchanged day 7). The semi-quantitative imaging-based scoring system identified the severity of mucositis as defined by histopathology. The combination of imaging criteria used allowed for the detection of early, intermediate and late mucositic changes. Imaging data gave higher scores compared to clinical scores early on, suggesting that the imaging-based diagnostic scoring was more sensitive to early mucositic change than the clinical scoring system. Once mucositis was established, imaging and clinical scores converged. Conclusion: OCT/ODT identified chemotherapy-induced oral changes prior to their clinical manifestation, and the proposed scoring system for oral mucositis was validated for the semi-quantification of mucositic change. Supported by TRDRP 14IT-0097, CRFA 30003, CCRP 00-01391V-20235, NIH (LAMMP) RR01192, DOE DE903-91ER 61227, NIH EB-00293 CA91717, NSF BES-86924. | ||
| Seq #222 - Periodontics, Endodontics, Soft tissues 3:30 PM-4:45 PM, Friday, March 23, 2007 Ernest N. Morial Convention Center Exhibit Hall I2-J | ||
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