0359 Effect of Blood Contamination on Microleakage of a Self-etching Adhesive
M. FATHI, Isfahan University of Technology, Iran, and V. MORTAZAVI, Isfahan University of Medical Sciences, Iran

Objectives: Introduction of materials and procedures that can reveal marginal seal in the situation of blood contamination has an important role in clinical success of a restoration. The purpose of this study was determination and comparison of microleakage scores of composite and compomer restorative materials with application of a self – etching adhesive, in the situation of blood contamination in class V restorations. Methods: One hundred and twenty class V cavities were prepared in dentino – enamel Junction of buccal and lingual surfaces of extracted human sound molars. After application of Prompt L –Pop (3M/ESPE) as an adhesive, the cavities were divided into 10 groups with 12 samples. Group Z1 was control group. Group Z2 was blood contaminated group. Group Z3 rinsed with water after contamination. Group Z4 rinsed with water after contamination and Prompt L-Pop reused. Group Z5 rinsed with Naocl after contamination and Prompt-L-Pop reused. The cavities were filled with composite (Z250, 3M/ ESPE). The remained five groups (F1 to F5) had just the same procedures but filled with compomer (F2000, 3M/ESPE). After polishing and thermocycling (×500), the microleakage scores of specimens were evaluated with dye penetration method. The data were statistically analyzed using Kruskal- Wallis and Mann- Whitney tests. Results: Kruskal-Wallis revealed statistically significant difference among composite groups in gingival margins (P=0.017). According to Mann-Whitney test group Z2 showed statistically significant higher microleakage scores than groups Z1, Z4, and Z5 in gingival margins (P=0.005, P=0.015 and p=0.015 respectively). Conclusion: According to the results of this study, blood contamination could increase the microleakage of composite and Prompt L-Pop in gingival margins. In this condition rinsing with water or Naocl and reusing Prompt L-Pop could be able to decrease microleakage effectively.

Seq #71 - Adhesion-microleakage
2:00 PM-3:15 PM, Thursday, March 22, 2007 Ernest N. Morial Convention Center Exhibit Hall I2-J

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