| 1165 Inhibitory Effect of Electrolyzed Oxidizing (EO) Water on Biofilm | ||
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T. KATO, A. YAMANAKA, K. YAMADA, R. KIMIZUKA, K. ISHIHARA, and K. OKUDA, Tokyo Dental College, Chiba, Japan Objective: Electrolyzed oxidizing (EO) water is effective against many microorganisms, and has been attracting attention due to its environmental safety. In this in vitro study, we investigated the antimicrobial activity of EO water against biofilms of oral bacteria and Candida albicans. Methods: Streptococcus mutans, Streptococcus sobrinus, Porphyromonas gingivalis, Prevotella intermedia, Actinobacillus actinomycetemcomitans, Fusobacterium nucleatum, and C. albicans were used in this study. Planktonic cells of each microorganism were treated with EO water for 15 and 60 sec. As a control, distilled water, acetic acid water (pH 2.5) and hydrochloric acid water (pH 2.5) were also used. Biofilms of S. mutans, S. sobrinus, A. actinomycetemcomitans, and C. albicans were prepared on a 96-well culture plate, and the effect of EO water on these biofilms was investigated. Biofilm was exposed to EO water for between 3 min to several hours. Biofilm formation was measured by the crystal violet staining method. Biofilm bioactivity was measured by the ATP measurement method. With the exception of C. albicans, the bacteria were further cultured following EO water treatment by addition of fresh broth to determine biofilm formation. Results: EO water killed the planktonic microorganisms within 15 sec. C. albicans biofilm and its ATP level decreased with EO water treatment, showing a significant decrease at 1 hr (p<0.01). Mutans streptococcus and A. actinomycetemcomitans biofilms showed no reduction with 3 min treatment, although the ATP levels started to significantly decrease at 15 sec after initiation (p<0.05). However, in some strains, no significant differences from the results of the controls were noted. In strains in which ATP levels were reduced, biofilm formation showed no increase, even though culture was continued. Conclusion: The results of the present study suggest that EO water is effective against some biofilms. | ||
| Seq #126 - Microbes and Oral Infections 3:30 PM-4:45 PM, Thursday, March 22, 2007 Ernest N. Morial Convention Center Exhibit Hall I2-J | ||
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