| 2382 Harakiri in Predicting Malignant Transformation of Oral Submucous Fibrosis | ||
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Y.-F. HUANG, S.-L. CHEN, and H.-W. YANG, Chung Shan Medical University, Taichung, Taiwan Objective: Apoptosis is a well-regulated cellular process inhibiting or activating the function of self-destruction in cells. Up to now, investigators have identified more than 100 genes whose expressions will affect the survival of the cells. Among those genes, Harakiri (Hrk) has been shown to bind an apoptosis inhibitor, Bcl-2, therefore making Harakiri a candidate for the induction of apoptosis. Methods: We retrieved 5 cases of oral submucous fibrosis and 7 cases of oral squamous cell carcinoma from the oral pathology service archive at Chung Shan Medical University. Immunohistochemistry was performed with a polyclonal antibody against human Harakiri. To study the expression of Harakiri at the translational level, we cultured, to confluence, 2 different oral squamous cell carcinoma cell lines, GNM, TSCCa, and primary human gingival fibroblasts. Whole-cell lysates were obtained for Western blot analysis. Results: Immunohistochemical staining showed that the expression of Hrk gradually decreased from the basal cell layer toward the superficial layer (5/5). However, the staining of Hrk was dramatically decreased in the tumor nests of well-differentiated squamous cell carcinoma (6/7). Preliminary Western blotting demonstrated that the expression of Hrk was reduced in 2 different oral squamous cell carcinoma cell lines (GNM, TSCCa), compared with primary human gingival fibroblasts. Conclusion: Reduced expression of Hrk in the tumor nests of oral squamous cell carcinoma suggested that Hrk may be a potential biomarker to predict the malignant transformation of oral submucous fibrosis. | ||
| Seq #240 - Premalignant Oral Lesions and Oral Cancer 3:30 PM-4:45 PM, Friday, March 23, 2007 Ernest N. Morial Convention Center Exhibit Hall I2-J | ||
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