| 2558 Inhibition of Sucrose-Dependent Multi-Species Microbial Biofilm Formation by Acarbose | ||
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R.P. NGUYEN, University of California - San Francisco, USA, and C.I. HOOVER, University of California - San Francisco, San Francisco, USA Introduction: Glucan formation from dietary sucrose by glucosyltranferases is a critical component of biofilm formation by cariogenic mutans streptococci (MS). Previous studies have shown that acarbose, an α-amylase inhibitor used to treat diabetes, inhibits glucosyltransferase activity and sucrose-dependent mono-species MS biofilm formation. Objective: Naturally-occurring oral biofilms are composed of multiple microbial species. Our goals were to determine if co-culture of selected oral bacteria with MS increased their ability to colonize biofilms and if acarbose inhibited formation of these sucrose-dependent multi-species biofilms. Methods: Mono-species and multi-species biofilms were formed on polystyrene tubes by culture (or co-culture) in BHI or BHI-0.3% sucrose broths. Multi-species biofilms contained MS, lactobacilli, and S. gordonii (SG) or S. sanguinis (SS). Following incubation, non-adherent bacteria were removed by pouring and washing the tubes with PBS. Biofilm, adhered to the tube walls, was quantified by crystal violet staining and the microbial composition determined by culture. Biofilms were formed in the presence and absence of acarbose to determine its inhibitory effect. Results: None of the species formed obvious biofilms in the absence of sucrose (OD560 (≤0.04). MS and SS formed significant sucrose-dependent mono-species biofilms (OD560 0.50-0.58) containing 6.9-8.3 log10 colony-forming units (p≤0.001), whereas lactobacilli and SG did not (OD560 ≤0.07, ≤5.5 log10). Co-culture of lactobacilli and SG with MS increased their biofilm colonization ~10-100-fold. These multi-species biofilms (OD560 0.30) contained 7.1 log10 MS, 7.3 log10 lactobacilli, and 6.5 log10 SG). Acarbose significantly inhibited (p≤0.02) multi-species biofilm formation (OD560 0.03; 5.4 log10 MS, 5.5 log10 lactobacilli, 5.2 log10 SG). Conclusions: MS and SS formed significant sucrose-dependent mono-species biofilm(s), whereas lactobacilli and SG did not. Co-culture of lactobacilli and SG with MS significantly increased their colonization of biofilm. Acarbose inhibited multi-species biofilm formation. These results indicate that acarbose has potential as an anti-plaque anti-caries agent. Support: NIH-NIDCR T32 DE07306-11 Marshall COHORT | ||
| Seq #267 - Junior Category 10:45 AM-12:00 PM, Saturday, March 24, 2007 Ernest N. Morial Convention Center Exhibit Hall I2-J | ||
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