| 2553 2-D Differential Analysis of Saliva Protein Markers in Sjögren's Syndrome | ||
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R.S. ATTIA, L.R. COOPER, M. CHOW, C.Q. NGUYEN, J.G. CORNELIUS, S. STEVENS, A.B. PECK, and M.E. DOYLE, University of Florida, Gainesville, USA Objectives:Sjögren's Syndrome (SjS), a human condition characterized by loss of exocrine function resulting from a chronic autoimmune attack primarily against the lacrimal and salivary glands, is well-represented by the NOD mouse model. Currently, no single diagnostic test exists that is specific for SjS;thus, new approaches are imperative. The objective of this study was to identify differences in saliva protein expression profiles during development and onset of SjS-like disease in NOD.B10-H2b mice versus disease-free CD1 mice as a means to find potential biomarkers. Methods: Salivas were collected from individual mice at various time-points between 4 and 32 weeks of age following intraperitoneal injections of an isoproterenol/pilocarpine mixture.Each age-pooled CD1 mouse saliva protein sample (4μg) was labeled with 400 pmoles Cy3 while each age-pooled NOD.B10-H2b mouse saliva protein sample (4μg) was labeled with 400 pmoles Cy5.The labeled samples were mixed and the proteins separated by 2D Fluorescence Difference Gel Electrophoresis (2-DIGE). Fluorescence images of CD1 and NOD.B10-H2b saliva protein maps were acquired with green (532nm) and red (633nm) lasers, respectively.Protein expressions were analyzed by Nonlinear Dynamic's Phoretix 2D Evolution software. Selected spots were identified by mass spec (MS). Results: Changes in expression, expression levels, and protein-processing have been identified for a number of proteins, not only between diseased and non-diseased animal salivas, but also during development and onset of SjS-like disease in NOD.B10-H2b mice. These gain/loss changes involve multiple classes of protein. Conclusion: Data have proven to be highly supportive of biochemical/pathophysiological changes known to occur during onset of SjS-like disease.In addition, several unique protein profiles appear temporally that need to be examined further as possible biomarkers for disease, first in this mouse model, then in SjS.Supported by a UFCD Student Research Fellowship (RA), Center for Orphaned Autoimmune Disorders (ABP), and NIDCR grant DE013769 (ABP) | ||
| Seq #267 - Junior Category 10:45 AM-12:00 PM, Saturday, March 24, 2007 Ernest N. Morial Convention Center Exhibit Hall I2-J | ||
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