| Seq #98 | Friday, 10 March 2006 | |||||||||||||||||||
| 9:00 AM-10:30 AM Dolphin Hotel No. Hemis E-2, Symposium - Group/Division Sponsored | ||||||||||||||||||||
| [AADR] Live and Static Imaging of Mineralized Tissues | ||||||||||||||||||||
Sponsored by: Craniofacial Biology, Mineralized Tissue, Periodontal Research, Pulp Biology | ||||||||||||||||||||
| Description: Bone is a dynamic, living organ that remodels to a greater degree than the majority of organs in the body. However it is extremely difficult to visualize bone cell activity, morphology, and gene expression due to its high mineral content. Recently breakthroughs have been made in molecular imaging in mineralized tissues. The goal of the symposium will bring together state of the art presentations addressing the technologies used for imaging of live and static mineralized tissues for the dental community. Jerry (Jian) Feng will address the static imaging approaches for visualization of bone and dentin, including 1) combining DAPI nuclear staining with fluorochrome labeling for visualizing the location of cells within mineralizing matrix; 2) Environmental scanning electron microscopy of acid-etched resin casted samples for visualization of osteocytes or dentin tubule structures; and 3) visualization by confocal microscopy of osteocyte or odontoblast processes and the organization of the peritubular system or osteocyte dendrites using Procion red dye injection. David Rowe will describe GFP reporter mice or primary osteoblast cultures in real time for cellular differentiation within the osteoblast lineage. In addition the multiplexing is used for interpreting host/donor contribution to tissue engineering and bone cell transplantation studies. Sarah Dallas will present dynamic time lapse imaging extracellular matrix assembly and reorganization of living osteoblasts using fluorescent probes for type I collagen, fibronectin and other bone extracellular proteins. These technologies have greatly advanced our understanding of cell differentiation and function in mineralized tissues. This mini-symposium on static and live imaging technologies will provide researchers with the latest information on powerful tools to study cell differentiation, changes in morphology, and dynamic relationships between cells and mineralized matrix using osteoblasts, osteocytes and dentin tubules | ||||||||||||||||||||
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Back to the ADEA/AADR/CADR Meeting & Exhibition (March 8-11, 2006)