| 0934 Platelet-Rich Plasma Inhibits Osteoinduction by DBM in Immunocompromised Mice | ||
|
D. RANLY1, C.H. LOHMANN2, D. ANDREACCHIO2, B.D. BOYAN1, and Z. SCHWARTZ1, 1Georgia Tech Research Institute, Atlanta, USA, 2University of Hamburg-Eppendorf, Germany Objectives: Clinical effectiveness of platelet-rich-plasma (PRP) is variable. To examine potential factors contributing to this variability, this study evaluated the effect of PRP on DBM-induced bone formation. Methods: PRP was prepared from blood drawn from six young males (Age=29±2.4 years). Platelet number was determined; growth factors were quantified before and after platelet-activation. Human demineralized bone matrix (DBM) from two donors (DBM-1, DBM-2) was mixed with 25-µl activated PRP and implanted bilaterally in the gastrocnemius muscle in male nude mice (n=8 implants/treatment group). On d-56 post-implantation, the hind-limb calf muscles were harvested for histology. Osteoinduction was evaluated by a qualitative score (QS) and morphometric measurements of ossicle size, new bone formation, and residual DBM. Results: The concentrating ability of the PRP preparatory system was clearly demonstrated: platelet count was amplified 4x over platelet-poor plasma; TGF-beta-1 was increased 15x, PDGF-BB 6x, PDGF-AA 5x, and PDGF-AB 2x. Under the conditions of this study, PRP reduced new bone formation by DBM, and the magnitude of this effect depended on the DBM donor. DBM-1 was more osteoinductive than DBM-2 based on greater ossicle area. The effects of the PRP ranged from neutral to inhibitory depending upon the DBM batch to which it was added. For DBM-1, PRP did not alter the QS or overall ossicle size, but it decreased new bone area. For DBM-2, PRP caused a reduction in all parameters measured. Moreover, it increased the amount of residual DBM. The effects on osteoinduction also varied with donor PRP. For example, the PRP from one donor did not inhibit ossicle area induced by DBM-1 but decreased the area in DBM-2 by 75%. Conclusion: PRP did not enhance the osteoinductivity of DBM when implanted in immunocompromised mice, and the activities of both DBM and PRP were donor dependent. (Supported by a grant from DePuy Germany.) | ||
| Seq #129 - Therapeutic Approaches for Periodontitis Management 10:45 AM-12:15 PM, Friday, 10 March 2006 Dolphin Hotel No. Hemis A-2 | ||
|
Back to the Periodontal Research - Therapy Program
| ||