Intracellular calcium is known to be important mediator for various cellular events. Thus fluoride dependent alterations in Ca-metabolism during odontogenesis can be crucial for the enamel-dentin mineralization and ameloblasts-odontoblasts survival. Calbindin D-28k (CB-D28k) known as the main cytosol buffer has been detected in various compounds of tooth-germ. That suggests its role for the local calcium homeostasis. Objectives:of our study was to investigate putative changes in CB-D28k expression in tooth-germs of rats supplemented with fluoride. Methods:21 pregnant rats, randomly divided into 3 groups were fed with standard diet and given: tap water (C-control), water with low (FL) and high (FH) fluoride concentration, respectively. 1-& 5-days-old pups were anesthetized, perfused, frozen, cut saggitally on cryostat and stained immunohistochemically. The quantitative data were confirmed with Western Blot. Results:Generally the localization of the positive immunoreactivity for CB-D28k did not differ between all the investigated groups regarding to the ameloblastic cells lineage. Surprisingly odontoblasts from the FH-groups were weakly positive as well. Western Blot revealed the tenfold increase in the amount of CB-D28k between 1- and 5-day-old FH-groups. Conclusions: It is likely that the supplementation of the high concentration of fluoride puts the developing tooth-germ under severe Ca-stress thus leads to the up-regulation of the expression of CB-D28k.

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