| 2479 Differentiation/proliferation of MG63 cells and human osteoblasts on titanium surfaces | ||
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A. SCHEDLE1, Z. QU2, X. RAUSCH-FAN1, M. MATEJKA1, and M. WIELAND3, 1Medical University of Vienna, University Clinic of Dentistry, Austria, 2Medical University of Vienna, University Clinic of Dentistry, Vienna, Austria, Dalian Stomatology Hospital, Dalian, China, 3Institut Straumann AG, Basel, Switzerland Objectives: The aim of this study was to investigate the influence of different implant surface topographies and chemistries on the expression of differentiation/proliferation markers on MG63 cells and primary human alveolar osteoblasts. Methods: Hydrophobic acid-etched (A) and coarse-grit-blasted, acid-etched (SLA) surfaces and hydrophilic acid-etched (modA) and modSLA surfaces were produced. Thereby, modA and modSLA surfaces were rinsed under nitrogen protection and stored in a sealed glass tube containing isotonic NaCl solution at pH 4-6. Tissue culture plates without specimens served as controls. The behaviour of MG63 cells and human osteoblasts grown on all surfaces was compared through determination of cell proliferation (3H-Thymidin incorporation, MTT colorimetric assay) and expression of osteocalcin (OC), osteoprotegerin (OPG), transforming growth factor-beta1 (TGF-ß1) and vascular endothelial growth factor (VEGF), detected in cell supernatants with ELISA-testkits Results: Proliferation of MG63 and primary cells was highest on controls, followed by A surfaces, mod A and SLA surfaces being almost on the same level and lowest on modSLA surfaces. modSLA surfaces exhibited highest alkaline phosphatase and osteocalcin production, followed by SLA, modA and A surfaces. Results with primary cells were comparable to the osteoblastic cell line MG63. Similar results were found for OPG, TGF-ß1 and VEGF production of primary cells, whereas for MG63 cells a slightly different rank order of surfaces was observed. ModSLA still showed the highest production of these molecules, but was followed by modA, SLA and A. Statistical significance was checked by ANOVA (p<0.05). Conclusion: MG63 cells and human primary alveolar osteoblasts show similar proliferation and differentiation characteristics on different titanium surfaces. Only modA surfaces showed enhanced expression of OPG, TGF-ß1 and VEGF on MG63 cells compared to primary human alveolar osteoblasts. Overall, the smallest proliferation rates and the highest expression of differentiation markers and growth factor production were observed on modSLA. Supported by ITI-grant 334/2003 | ||
| Seq #200 - Cell Culture & Bone Formation 8:00 AM-10:00 AM, Saturday, 1 July 2006 Brisbane Convention & Exhibition Centre M1 | ||
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