Objective: Using PIGE (proton induced gamma-ray emission)-method (TIARA, JAPAN), we have studied the fluoride (F) distribution in human tooth under various circumstance. We report here F uptake into the human tooth from the thin layer of F-releasing material (FRM). Methods: The crown of human tooth was horizontally cut. The dentin of cut surface was firstly applied with FRM about 50~100μm in thickness (three kinds of bonding agent and one adhesive liner), then F-releasing filling materials (FRFM, 1.5mm in thickness) were applied on the thin layer of FRM as the instruction of manufactures: G1-A; FL-Bond and BEAUTIFIL (SHOFU), G2-A; REACTMER BOND and REACTMER PASTE (SHOFU), G3-A; XENO Bond and XENO CF PASTE (Sankin), and G4-A; PROTECT LINER F and TEETHMATE F-1 (KURARAY). As control, non-FRFM (LITE FIL IIP, SHOFU) about 1.5mm in thickness was applied on the thin layer of FRM. : G1-B; FL-Bond and LITE FIL IIP, G2-B; REACTMER BOND and LITE FIL IIP, G3-B; XENO Bond and LITE FIL IIP and G4-B; PROTECT LINER F and LITE FIL IIP. The treatment and the measurement of specimens are as same as previously reported (Nucl. Instr. And Meth.B 190 2002). The F uptake from FRM after one month was estimated in the form 2D map. Results: F distributions from FRM in G1 and in G4 were different between with or without FRFM. In G1-A and G1-B case, for example, the maximum F concentration was 1120~1140(mean=1130) ppm in G1-A, 490~790(mean=650) ppm in G1-B, and the F penetration depth was 72~135(mean=110)μm in G1-A, 20~34(mean=29)μm in G1-B (P<0.05). Conclusion: F uptake from thin layer of FLM depends on the kinds of materials. This may suggest the necessity to study F uptake from the thin layer of FRM after longer time than one month.

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