| 2321 Mutans Group Streptococci: Maximum Inhibitory Dilution (MID) of Mouthwashes | ||
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E. WATANABE, A.P. NASCIMENTO, M.P. VERRI, J.M.G. TANOMARU, F. MATOBA-JÚNIOR, and I.Y. ITO, Faculdade de Ciências Farmacêuticas de Ribeirão Preto - USP, Ribeirão Preto, SP, Brazil Objectives: To compare in vitro Maximum Inhibitory Dilution (MID) of 4 mouthwashes with different active ingredients against mutans group streptococci (MGS). The products analyzed were Listerine® containing essential oils, Periogard® containing chlorhexidine, Cepacol® containing cetylpyridinium chloride and Plax® containing triclosan. Methods: The antimicrobial activity was realized in 96-well microtiter plates against 36 field MGS isolated from 26 individuals. Each mouthwash was submitted to a serial twofold dilution (1/2.5 to 1/2480) using double concentration of tryptose soy broth with 1.0% yeast extract. The final volume in each well was 100.0ml plus 5.0ml of a microbial suspension equivalent to a 0.5 McFarland scale. The incubation was realized anaerobically at 37ºC for 48 hours and the antimicrobial activity analyzed by stereomicroscope under reflect light, considering MID as the highest dilution of products that inhibited the microbial growth. Results: From 36 field MGS, 26 (72.2%) were Streptococcus mutans and 10 (27.8%) Streptococcus sobrinus. Listerine® had no antimicrobial activity for any dilution against any of 36 strains; Periogard® revealed antimicrobial activity against 1, 2, 27, 35 and 36 strains in dilutions of 1/5120, 1/2560, 1/1280, 1/640 e 1/320; Cepacol® inhibited the growth of 2, 23, 35 and 36 strains in dilutions of 1/2560, 1/1280, 1/640, 1/320 and Plax® had antimicrobial activity against 2, 27, 28, 36 strains in dilutions of 1/640, 1/320, 1/160 and 1/20. The results submitted to statistical analysis using Kruskal-Wallis test revealed that MID of Periogard® was not different of Cepacol® (p>0.05) being higher than Plax® (p<0.05) which was better than Listerine® (p<0.05). Conclusions: Mouthwashes containing chlorhexidine and cetylpyridinium chloride had higher antimicrobial activity than mouthwash containing triclosan. Moreover, mouthwash containing essential oils was not able to inhibit MGS growth. | ||
| Seq #187 - Streptococci 3:30 PM-4:30 PM, Friday, 30 June 2006 Brisbane Convention & Exhibition Centre Exhibit Hall 1 | ||
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