| 1995 Epithelial-specific receptor expression using a rat periodontitis model | ||
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E. PUTNINS, University of British Columbia, Vancouver, Canada, D. EKUNI, Okayama University, Japan, and J.D. FIRTH, University of British Columbia, Faculty of Dentistry, Vancouver, Canada Objectives: Regulation of epithelial cell behavior associated with periodontitis is not well elucidated but many responses will ultimately be regulated by growth factor receptors. This study examined junctional and pocket epithelium protein and gene receptor expression using a rat experimental periodontitis model. Methods: Periodontal disease was induced by daily topical application of lipopolysaccharide and protease using an established protocol. Animals were sacrificed at time zero (control), 2 and 8 weeks. Frozen tissue samples were collected from right palatal gingival soft tissue and the left periodontal tissues were decalcified and embedded in paraffin. Laser microdissection and real-time RT-PCR was used to quantify keratinocyte growth factor receptor (KGFR), hepatocyte growth factor receptor (HGFR), epidermal growth factor receptor and fibroblast growth factor receptor 1 gene expression and in situ RT-PCR localized these increases to specific epithelial cells. Receptor protein expression was examined immunohistochemically. In cell culture, induction of HGFR and KGFR protein expression by serum, lipopolysaccharide and proinflammatory cytokines were examined using flow cytometry. Results: Eight week tissue samples exhibited histological changes consistent with periodontitis. KGFR and HGFR gene and protein expression were significantly induced from health to disease. KGFR expression was significantly upregulated in basal and parabasal pocket epithelial cells but HGFR was upregulated throughout the pocket epithelium. In cell culture serum, lipopolysaccharide and proinflammatory cytokines, IL-1β and TNF-α, significantly induced KGFR protein receptor expression but HGFR expression was only induced by serum. Conclusion: KGFR and HGFR are highly upregulated with the onset of periodontitis and may possibly play a significant role in regulating proliferation and migration of pocket epithelium. | ||
| Seq #165 - Host Responses and Gene Expression 1:30 PM-3:30 PM, Friday, 30 June 2006 Brisbane Convention & Exhibition Centre P4 | ||
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