Polymorphism of Glucokinase gene and association with NIDDM and DRS

*1455 Polymorphism of Glucokinase gene and association with NIDDM and DRS*
Z.N. AL-DWAIRI¹, W. COULTER², I. BENINGTON², and P.L. HYLAND², ¹Jordan University of Sciences and Technology, Irbid, Jordan, ²Queens University, Belfast, United Kingdom Introduction: Recent advances in molecular genetics have allowed recognition of the genes involved in some types of Non-insulin dependent diabetes mellitus (Maturity Onset Diabetes of the Young). Despite these successes, little is known about susceptibility genes to the common forms of NIDDM. Glucokinase (GCK), the major enzyme responsible for glucose phosphorylation has been considered a prime candidate for inherited defects predisposing to NIDDM and polymorphic markers, GCK1, GCK2 that flank the gene have been identified. Objective: The aim of this study was to investigate the association between NIDDM and GCK1 microsattelite polymorphism and denture-related stomatitis (DRS) in Northern Ireland population. Methods: This study involved 30 subjects with late-onset diabetes mellitus; of whom 24 out of 30 were affected by DRS, and 30 control non-diabetic controls; 15 of whom had DRS. DNA was extracted and purified from buccal epithelial cells, polymerase chain reaction carried out, followed by gel electrophoresis and direct cycle sequencing. Results: A dinucleotide CA- repeat of the sequence (CT)4 TTT GT(CT)7 (CA)9 AA(CA)4CCACATA (CA)3 was identified approximately 10Kb 3' of GCK gene with two different alleles: Z allele (195 bp) and Z+4 allele (199 bp). Z+4 was identified in 40% of diabetic subjects compared to 13% of controls. Normal Z allele was identified in 60% in diabetics compared to 87% among controls. There was no significant association between GCK1 polymorphism and level of glycated hemoglobin (p<0.05). The result showed that Z+4 allele was associated significantly with the presence but not the severity of denture-related stomatitis in the diabetic subjects Conclusion: This study demonstrates a relation ship between GCK1 gene polymorphism and NIDDM in this ethnic group. It is concluded that Glucokinase microsattelite, which may segregate GCK gene, is a useful marker for identification of susceptibility to late onset diabetes mellitus and the presence of DRS.
Seq #117 - Oral Diagnosis and Epidemiology 3:30 PM-4:30 PM, Thursday, 29 June 2006 Brisbane Convention & Exhibition Centre Exhibit Hall 1
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