| 2475 In vitro optimizing surface topography of zirconia and alumina abutments | ||
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K. MUSTAFA, University of Bergen, Bergen, Scandinavian Institute of Dental Materials (NIOM), Oslo, Norway, A. WENNERBERG, University of Goteborg-, Sweden, K. ARVIDSON, University of Bergen, M. KHADRA, University of Oslo - Dental Faculty, Norway, P. HAAG, Nobel Biocare AB, Goteborg, Sweden, and S. KARLSSON, Scandinavian Institute of Dental Materials (NIOM), Oslo, Norway Objectives: As new implant materials are introduced to obtain a sufficient soft tissue barrier, the aim of this study was to determine effect of surface modifications of zirconia and alumina on attachment and proliferation of human gingival fibroblasts. Methods: Fibroblasts were cultured on discs of pressed sintered alumina or zirconia, and then milled (1 & 2), pressed, alumina or zirconia and then polished (3 & 4), pressed, alumina and then veneered with NobelRondo baseliner al (5), pressed, zirconia and then veneered with Cercon-S baseliner (6), and alumina or zirconia discs were veneered with the baseliners and then polished (7 & 8). The different surfaces were analyzed by optical interferometer and SEM. Cell profile areas were measured by an image analyzer. Cell attachment was determined after 3 and 24 hours as a ratio of the cell profiles and the total micrograph area and was expressed as percent of attachment. MTT analyses were performed to determine cellular attachment after 3 hours of incubation and cellular proliferation after 7 days. Results: The zirconia polished discs had the smoothest surface in terms of average height deviation (Sa = 0.03 µm), the roughest were the milled zirconia surface (Sa = 0.36 µm). The application of baseliners created smoother surfaces with Sa value of 0.06 µm in alumina (NobelRondo) and 0.12 µm in zirconia (Cercon-S). The milled surfaces on both alumina and zirconia had significantly higher percentages of initial cell attachment and proliferation compared to the other surfaces. Fibroblasts attached and grown effectively on the surfaces veneered with NobelRondo baseliners through out the experiments. Conclusions: Although the roughness of all the surfaces investigated in this study were less than 0.4 µm, there were significant differences in cellular attachment and proliferation demonstrating in vitro the important role of the surface microstructure of ceramic abutments. | ||
| Seq #200 - Cell Culture & Bone Formation 8:00 AM-10:00 AM, Saturday, 1 July 2006 Brisbane Convention & Exhibition Centre M1 | ||
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