| 0813 Reuptake of Extracellular Amelogenin by Dental Epithelial Cells | ||
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A. TANIGUCHI, National Institute for Materials Sceinces, Biomaterials Center, Tsukuba, Japan, L. XU, Natinal insutitute for materials sciences, Biomaterials center, Tsukuba, Japan, and H. HARADA, Osaka University Graduate School of Dentistry, Japan Objectives:Amelogenin is an extracellular matrix protein secreted by ameloblasts and is a major component of enamel matrix. Recently, in addition to their role in enamel formation, the biological activity of enamel proteins in the process of cell-differentiation has recently become widely appreciated. In this study, we examined biological activity of amelogenin on ameloblast differentiation. Methods: We expressed a mouse amelogenin (rmAmelogenin) by baculovirus-insect cell expression system The differentiation-related marker gene expression was determined by real-time PCR method. Results:Recombinant mouse amelogenin enhanced expression of endogenous amelogenin mRNA in a cultured dental epithelial cell line (HAT-7), despite of a lack of increased amelogenin promoter activity. The half-life of amelogenin mRNA is extremely short, but in the presence of rmAmelogenin its half-life was extended four times longer than control. Furthermore, we showed entry of exogenous FITC-conjugated rmAmelogenin into the cytoplasm of HAT-7 cells.Conclusions: It follows from our results that exogenous amelogenin increases amelogenin mRNA levels through stabilization of mRNA in the cytoplasm of HAT-7 cells. | ||
| Seq #70 - Mechanisms of Odontogenesis 11:00 AM-12:00 PM, Thursday, 29 June 2006 Brisbane Convention & Exhibition Centre Exhibit Hall 1 | ||
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