| 2476 Influence of hydrophilic versus hydrophobic implant surfaces on MG63 cells | ||
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Z. QU1, X. RAUSCH-FAN2, M. WIELAND3, M. MATEJKA2, and A. SCHEDLE2, 1Medical University of Vienna, University Clinic of Dentistry, Vienna, Austria, Dalian Stomatology Hospital, Dalian, China, 2Medical University of Vienna, University Clinic of Dentistry, Austria, 3Institut Straumann AG, Basel, Switzerland Objectives: Hydrophilic modified coarse-grit-blasted, acid-etched (mod SLA) surface showed enhanced bone formation in vivo and osteoblast phenotypic expression in vitro. The aim of this study was to investigate the mechanisms of the influence of different implant surface topographies and chemistries on proliferation, migration, differentiation and cluster formation of the osteoblastic cell line MG63. Methods: Hydrophobic acid-etched (A) and coarse-grit-blasted, acid-etched (SLA) surfaces and hydrophilic acid-etched (modA) and modSLA surfaces were produced. Thereby, modA and modSLA surfaces were rinsed under nitrogen protection and stored in a sealed glass tube containing isotonic NaCl solution at pH 4 to 6. The behaviour of MG63 cells grown on all surfaces was compared through determination of cell attachment (3H-Thymidin incorporation) and proliferation [cell counting kit-8 (Dojido Laboratories, Japan)], time-lapse microscopy of fluorescence labeled cells (cell tracker orange, Molecular Probes, USA) and determination of gene expression by reverse transcription-polymerase chain reaction (RT-PCR). Results: No significant difference of cell attachment was found. Increased cell proliferation was observed on A and SLA surfaces compared to modA and modSLA surfaces. After 2 days of incubation on modSLA and modA surfaces the formation of cell clusters has been observed by time-lapse microscopy. Thereby, cell clusters on modSLA surfaces were more pronounced and their size gradually increased with increased incubation time up to nine days, being larger than on modA surfaces throughout the whole incubation period. On A and SLA surfaces cells spread homogenously after two days, but cell cluster formation started after 4 days. The expression level of the bone-associated genes (alkaline phosphatase; osteocalcin; type I Collagen; osteoprotegrin; Gyleraldehy-3-phosphate dehydrogenase) detected by RT-PCR was highest on the modSLA surface (p<0.05; ANOVA). Conclusion: It has been demonstrated that the modSLA surface shows an early cluster formation of MG63 cells and an enhanced expression of key osteogenic regulatory genes. Supported by ITI-grant 334/2003 | ||
| Seq #200 - Cell Culture & Bone Formation 8:00 AM-10:00 AM, Saturday, 1 July 2006 Brisbane Convention & Exhibition Centre M1 | ||
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