Objectives: In prosthodontic treatment, the application of magnets could increase the retention of denture. Magnets could not only offer force control and treatment convenience, it could also increase the rate of tooth movement in orthodontic treatment. Recent studies suggest that SMF affect osteoblastic maturation by reducing the proliferation of the cells. However, the mechanism of osteoblast cell cycle change remains unclear. Our objective is to determine how the static magnet affects osteoblast differentiation. Methods: MG63 osteoblast-like cells were utilized for all in vitro tests. The cells were divided into the control and SMF-exposed groups. SMF effects on the proliferative activity of MG63 cells were evaluated using haemocytometers. PGE2 in the MG63 cell layer was determined with a multilabel plate reader. The plasma membrane fluidity of SMF treated cells was determined by measurement of the fluorescence anisotropy of hydrophobic fluorescent probe,1-(4-(trimethylammonium)phenyl)-6-phenylhexa-1,3,5-triene (TMA-DPH) Results: The number of cell doublings for the SMF exposed groups was significantly lower than that of the control group, varying in a flux density-dependent manner in the first 24-hr period (p<0.05). The MG63 cells exposed to SMF had greater released a greater amount (p < 0.05) of PGE2 than the cells with sham exposure after 12-hour. At 4 hrs, MG63 cells exposed to 0.4 T SMF exhibit a statistically significant increase in fluorescence anisotropy (increased from 0.13 to 0.16, p<0.05), indicating that SMF exposure reduced the fluidity of the cell membrane in the hydrophilic region. Conclusion: Based on these findings, this study suggest that SMF affect osteoblastic maturation by increasing the membrane fluidity and reducing the proliferation-promoting effects of growth factors at the membrane domain.

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