Seq #174 Friday, 11 March 2005

10:45 AM-12:45 PM Baltimore Convention Center 308, Symposium - Group/Division Sponsored
The Role of Genomics and Proteomics in Better Understanding Periodontal Regeneration

Sponsored by: Mineralized Tissue, Oral Medicine & Pathology, Periodontal Research
Description: The purpose of this symposium is to bring together scientists to provide the dental community with genomic and proteomic developments in the field of periodontal healing. Four speakers will take part of this symposium. Mark Bartold will address the proteomics of Periodontal Stem Cells. Through an understanding of the proteomic profile of cells residing within the periodontium, and in particular periodontal stem cells, unique markers for these cells along with a better understanding of the protein profile of these cells and their unique extracellular matrices will be presented. Bruce Rutherford will address cementoblast transcriptome and proteome. Several natural and experimental mutations in key regulators of phosphate metabolism reveal that local extracellular phosphate levels influence cementogenesis and the function of the periodontium. To study this phenomenon, the effect of extracellular phosphate concentration on the cementoblast transcriptome and proteome, isolated from normal and mutant mice was investigated. Understanding the role of phosphate in the normal development and function of cementum will inform approaches to periodontal tissue regeneration. Dana Graves will address use of integrated approaches in genomics and proteomics to identify mechanisms involved in complex cellular processes. The model of work here is TNF-Induced Apoptosis and two different approaches will be presented to demonstrate that the integrated use of proteomics and genomics can identify and functionally establish the role of transcription factors in cellular processes. Salomon Amar will address the influence of blockers of proinflammatory mediators in periodontal wound healing. A proteomic approach was taken to investigate protein changes in response to the influence of IL-1 and TNF blockers. Integrated maps of signal pathways as well as extracellular proteins affected by these modulations will be presented. Altogether the symposium speakers will highlight findings that have important ramifications for better understanding of normal, pathologic and regenerative activities of periodontal tissue
 
1429  10:45 AM Use of integrated approaches in Genomics and Proteomics to identify Mechanisms Involved in Complex Cellular Processes
D.T. GRAVES, Boston University, MA, USA
  11:00 AM Influence of blockers of proinflammatory mediators in periodontal wound healing
S. AMAR, Boston University, MA, USA
  11:15 AM Proteomics of Periodontal Stem Cells
P.M. BARTOLD, University of Adelaide, Adelaide SA, Australia
  11:30 AM Cementoblast transcriptome and proteome
R.B. RUTHERFORD, Dentigenix, Seatle, WA, USA

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