| 3539 Microbiota of Initial Periodontitis in Adults | ||
|
A. TANNER1, S.C. LU1, E. KANASI1, R.L. KENT, Jr.2, T.E. VAN DYKE3, and S. SONIS4, 1The Forsyth Institute, Boston, MA, USA, 2The Forsyth Institute, Harvard University, Boston, MA, USA, 3Boston University, MA, USA, 4Brigham & Women's Hospital, Boston, MA, USA Objectives: This study sought to compare the microbiota of periodontally healthy adults with subjects with early periodontitis, including sites with progressing attachment loss. Methods: 210 medically healthy subjects with minimal periodontal attachment loss, 20-40 yr, were monitored using full mouth clinical measures at 6-monthy intervals for 18-mos. Subjects were divided into healthy (mean AL ≤ 1.5mm, no sites > 2mm AL) and early periodontitis (Mean AL, >1.5 mm, ≥1 site 2 mm AL). Subjects with periodontitis progression had sites that lost ≥ 1.5 mm AL during monitoring. The subgingival microbiota of molar sites was analyzed using whole genomic DNA probes 40 to subgingival species, and PCR to P. gingivalis and T. forsythensis. Results: 44% subjects were male, 9% Asian, 17% African American, 7% Hispanic and 63% Caucasian. Cross-sectional analysis indicated that early periodontitis was associated with subject age, and PI, GI, BOP, and smoking compared to health. Of 113 subjects that completed 18-mo monitoring, 19% had increased AL loss >1.5-mm at interproximal sites. Progressing periodontitis was associated with elevated GI and PD compared to non-progressing subjects. There were few differences in the microbiota of subgingival molar sites between periodontal health and early and progressing periodontitis. Species associated (p<0.05) with baseline early periodontitis included E. saphenum, E. timidum, and A. actinomycetemcomitans by DNA probe and P. gingivalis and T. forsythensis by PCR. P. gingivalis (PCR) was associated with subjects with progressing sites. Conclusions: Periodontal attachment loss in cross-sectional and longitudinal analyses in subjects with minimal periodontitis was associated with gingival inflammation and pocket depth. Species associated with initial periodontitis included species associated with more advanced disease, particularly P. gingivalis and T. forsythensis. Supported by NIDCR Grant DE-09513. | ||
| Seq #370 - Markers, Risks, and Microbes 2:00 PM-4:00 PM, Saturday, 12 March 2005 Baltimore Convention Center Exhibit Hall E-F | ||
|
Back to the Periodontal Research - Diagnosis / Epidemiology Program
| ||