0924 Oral Health Improvement Using a Natural Tooth Cleaning Product
P. LAPEZACK1, C. MACHADO1, G.T. MILANO1, E.B. DOS SANTOS1, A.C.R. CHIBINSKI1, V.A. KOZLOWSKI, Jr.1, and J.M. GOODSON2, 1Ponta Grossa State University, Brazil, 2The Forsyth Institute, Boston, MA, USA

Introduction: Alternative methods for oral cleansing are used in different parts of the world and have received a special attention because of their application in poverty areas and potential use for populations with social-economic disabilities. Objectives: The purpose of this study was to evaluate the clinical and microbiological effects of tooth cleaning using a vegetable sponge (Luffa cylindrica).Methods:Following institutional ethics committee review and approval, fifteen children (10-12 years) participated in a supervised clinical trial over a five day period. The condition of their gingiva was evaluated using the gingival bleeding index (Ainnamo & Bay, 1974) and tooth deposits were scored according to the simplified debris index (Green & Vermillion, 1964). After initial evaluation, the children received supervised tooth cleaning with a vegetable sponge three times daily for 5 days. Before clinic evaluation, after day 2 and again after 3 days, saliva and natural sponge samples were collected for microbiologic evaluation. The children were reexamined at 5 days. All clinical examination and tooth cleaning supervision was performed by one examiner. Examiner reproducibility was determined by duplicate measurements for each subject. Results:There were no significant differences in the debris scores between day 0 and day 5 (p=0.36; Chi-square test). The gingival bleeding index scores were significantly lower on day 5 (p=0.009;Fisher test ). Saliva Candida UFC/ml counts were not different after 5 days (p=0.88;Student test ). Bacterial growth on natural toothbrush samples (UFC/ml) after day 2 (=1.95x108) and day 3 (=1.86x108) were not statistically different (p=0.84). The Lactobacillus UFC/ml count in saliva samples diminished after 5 days (126.54 to 23.22; p=0.15). Conclusions:Tooth cleaning using the natural product (Luffa cylindrica) demonstrated a significant effect by reduction in bleeding scores. The potential problem of bacterial growth in the natural sponge after tooth cleaning can be controlled by disposal after each use.

Seq #117 - Malodor, Children, Cancer, Oral Hygiene, Dental Sealants
2:00 PM-4:00 PM, Thursday, 10 March 2005 Baltimore Convention Center Exhibit Hall E-F

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