| 2284 Establishment and immunohistochemical analysis of the alveolar critical size defect | ||
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J.N. JAMES, University of Texas - Houston/Health Science Center, Rice University, USA, S. YOUNG, University of Texas - Houston/Health Science Center, USA, and A. MIKOS, Rice University, Houston, TX, USA Increasing demand for esthetic restoration, and tooth preservation have initiated numerous studies pertaining to dental surgeries, including surgeries involving the supporting bone of the dentition. These surgeries may require bone augmentation. Synthetic alloplasts show promise in eliminating invasive donor-site graft surgeries. Our aim has been the development and characterization of biocompatible and biodegradable polymer scaffolds that are able to interact with surrounding tissues and cells through biomolecular recognition, in a so-called “biomimetic” fashion. Objectives: To examine such a material, a test model must be constructed. Through the implantation of biomimetic scaffolds, we wish to recapitulate normal healing events in a critical size defect in order to achieve bone regeneration. Specifically, aims of this study include the development and characterization of a critical size defect in alveolar bone, and characterization of vessel formation within the critical size defect using immunohistochemistry, since the establishment of an adequate vascular bed is a prerequisite to osteogenesis. Methods: A 6.1mm defect was created on the facial surface of the mandible on both right and left sides of the rabbit. The animals were sacrificed at 2, 4, 8, and 16 weeks for histological analysis. The endothelial surface marker CD31 was chosen as a target antigen for IHC, in which a primary mouse anti-human CD31 antibody (DakoCytomation USA) was utilized to bind the surface antigen. Results: Osteoid formation was noted at 2 weeks post-operatively, and complete bony formation was detected at 8 weeks. No significant staining was detected in the preliminary study. Conclusions: A new study is in development wherein the location and geometry of the defect will be altered. The new study will test different fixation and decalcification methods as well as confirming positive staining on soft tissue of a rabbit using the same antibody. This work was in part supported by NIH T32 DE015355. | ||
| Seq #248 - Reconstruction, Wound Healing 2:00 PM-4:00 PM, Friday, 11 March 2005 Baltimore Convention Center Exhibit Hall E-F | ||
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