| 2282 Toxicity of a Novel Lysine Derived Polyurethane Adhesive | ||
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S. ABOD, University of Pittsburgh, PA, USA, and M.J. BUCKLEY, University of Pittsburgh, PA, USA Objective: The tissue sealant/tissue adhesive market is a vibrant and dynamic sector with emerging technologies, innovative concepts, and cutting-edge applications. The primary purpose of these projects is to facilitate the wound-healing process, primarily by improving on wound closure and hemostasis. The closure of both traumatic and surgical wounds are complicated by delayed healing, due to continued hemorrhage, development of hematomas, wound breakdown, and post-operative scarring due to poor tissue apposition. We have developed a lysine-derived urethane tissue adhesive that is biocompatible, resorbable... Our lysine-derived urethane tissue adhesive material exhibits a molecular structure that allows for various alterations, and is hence a technology platform that can be developed in many forms with a variety of physical properties. It can be used as an appropriate tissue adhesive or sealant with biomechanical strength to adhere to tissue with a watertight seal for a substantial period of time prior to bioresorption. As with any new material, toxicity testing is critical to the safety process. Methods: An in vitro cytotoxicity testing for biocompatibility was done using the ISO method from 1X MEM extract. An extract of the adhesive after cure was prepared using MEM supplemented with 5%serum and 2%antibiotics. The test extract was then placed onto a confluent monolayer of L-929 mouse fibroblast cells propagated in 5%CO2. . A reagent, positive and negative control were used. All monolayer were incubated at 37O in the presences of 5% CO2 for 48 hours. The monolayer was examined microscopically to determine cell morphology changes or cell lysis. Results: All test samples, reagent and positive controls showed 0% cell morphology changes (rounding), intracytoplasmic granules, or cell lysis. Conclusion: Under the conditions of this study, the 1X MEM test extract showed no evidence of causing cell lysis or toxicity.
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| Seq #248 - Reconstruction, Wound Healing 2:00 PM-4:00 PM, Friday, 11 March 2005 Baltimore Convention Center Exhibit Hall E-F | ||
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