| 0861 Initial cell response to functionally graded hydroxyapatite coating | ||
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N. OH1, B. THOMAS2, Y. YANG3, S. OH4, J.J. CUOMO2, A. RABIEI2, and J. ONG3, 1Inha University, University of Tennessee Health Science Center, Incheon, South Korea, 2North Carolina State University, Raleigh, USA, 3University of Tennessee Health Science Center, Memphis, USA, 4University of Tennessee, Memphis, USA Objective: The aim of this study was to evaluate the initial cell response to functionally graded hydroxyapatite (FGHA) coating produced using ion beam sputter deposition. Materials and Methods: FGHA coating were prepared using ion beam sputtering. The coating has a graded crystallinity and graded grain size across the thickness of the coating. The interface with the substrate had larger grains and higher crystallinity, whereas the outermost surface was amorphous and had smaller grains. The surface of HA coating was characterized using grazing angle x-ray diffraction. ATCC CRL 1486 human embryonic palatal mesenchymal cell, an osteoblast precursor cell line, was used to evaluate the initial cell adhesion and cell differentiation on HA coatings. For the cell adhesion study, cells were seeded onto the HA coating in 6 well culture plate at a density of 50,000 cells/sample in Dubecco Modified Eagle's Medium (DMEM) and incubated. After 0.5, 1, 2, 3, and 4 hour incubation, the non-adherent cells were removed and counted using Coulter Counter. The cell adhesion was calculated by subtracting the non-adherent cells from initial cell suspension. Total protein production and alkaline phosphatase production were measured at 4 days after cell confluence. Results: XRD pattern shows that the outermost layer of HA coating was amorphous. It was observed that cell adhesion on HA coatings remain plateau after 2 hours incubation. In addition, the cells on HA coating exhibited significantly less total protein production, but significantly greater alkaline phosphatase specific activity as compared to controls (p=0.00042), suggesting the enhancement of osteoblast differentiation. Conclusion: It was concluded that the FGHA coating exhibited good biocompatibility with respect to initial cell response and enhanced osteoblast differentiation as compared to the controls. | ||
| Seq #111 - Calcium Phosphate Coatings - Characterization and Cell Response 2:00 PM-4:00 PM, Thursday, 10 March 2005 Baltimore Convention Center Exhibit Hall E-F | ||
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