| 1245 Expression of leukocyte adhesion molecules on the lingual lymphatic endothelium | ||
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S.-I. KUROSHIMA1, Y. SAWA1, Y. YAMAOKA2, S. YOSHIDA3, and N. INOUE1, 1Hokkaido University, Sapporo, Japan, 2Hokkaido university, Sapporo, Japan, 3Hokkaido University, Kita-ku, Sapporo, Japan Objectives: The lymphatic endothelium is known to express platelet-endothelial cell adhesion molecule-1 (PECAM-1). We have previously reported that the lymphatic endothelium expresses intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in inflamed human small intestines. This study investigated the expression of PECAM-1, ICAM-1, and VCAM-1 on mouse lingual lymphatic vessels expressing a new lymphatic endothelial marker, podoplanin. Methods: The closed colony ICR mice with injections of TNF-alpha into the apex linguae, and of saline without TNF-alpha (sham) into the radix linguae were used to examine the expression of leukocyte adhesion molecules by rat anti-mouse PECAM-1, ICAM-1, and VCAM-1. The lymphatic vessels were identified by anti-mouse podoplanin. The sections were examined by laser-scanning microscopy, and confocal fluorescence images of optical slices on the Z-axis were acquired at 0.5-micrometer intervals. The digital images were processed by a Zeiss LSM Image Browser version 3.0 (Carl Zeiss). Results: The lymphatic endothelium expressed both PECAM-1 and ICAM-1, but not VCAM-1 like the blood endothelium. In tissue with hyper cellular infiltration and vasodilation with TNF-alpha the lymphatic endothelium expressed both PECAM-1 and ICAM-1, but not VCAM-1, whereas the blood endothelium expressed all of these. Collecting lymphatic vessels expressing ICAM-1 were observed in the muscularis, and further, there were more lymphatic vessels expressing ICAM-1 in the tissue with TNF-alpha than in normal tissue. Conclusions: These suggest that the ability to produce diverse leukocyte adhesion molecules in the lymphatic endothelium of oral mucosa may be weaker than in the intestinal lymphatic endothelium, and that inflamed lymphatic vessels may function to allow more immunocytes to migrate from tissue into the vessels than the population migrating under normal conditions | ||
| Seq #137 - Cancer Discoveries 2:00 PM-4:00 PM, Thursday, 10 March 2005 Baltimore Convention Center Exhibit Hall E-F | ||
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