| 1390 Micro-mechanics of the Porcine TMJ Using Scanning Acoustic Microscopy | ||
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E. BENAVIDES1, P. SPENCER1, J.L. KATZ1, B. FRICKE1, Y. WANG1, M. BILGEN2, and E. GLEESON3, 1University of Missouri -Kansas City, USA, 2University of Kansas Medical Center, Hoglund Brain Imaging Center, Kansas City, USA, 3Rice University, Houston, TX, USA Scanning acoustic microscopy (SAM) is a powerful experimental technique for non-destructive determination of the micro-mechanical properties of materials at micrometer resolution. A distinct advantage of this technique for the measurement of micro-mechanical properties of biological tissues is the liquid couplant used to transmit the acoustic signal from the lens to the sample prevents desiccation and heat generation of the specimen during measurement. OBJECTIVE: To measure in a non-destructive manner the in-vitro micro-mechanical properties of the tissues which comprise the neonatal porcine TMJ. METHODS: Using a Kraemer WINSAM 100 scanning acoustic microscope (Kraemer Scientific CO., Herboron, Germany) equipped with a 30 MHz lens, the acoustic impedance (AI) and modulus of elasticity (E) of the porcine mineralized and demineralized condyle, the demineralized temporal bone, and the disc were determined. These TMJ tissue properties were obtained either by extrapolation (for the collagenous tissues) or interpolation (for the mineralized tissues) from calibration curves based on SAM data for known polymeric and high AI materials. The results from the acoustic images were correlated with density distribution maps measured using magnetic resonance microscopy. RESULTS: The modulus of elasticity of the TMJ disc was calculated by extrapolation to be 3.6 GPa. The modulus of elasticity of the demineralized condyle and demineralized temporal bone was 3.7 GPa for both tissues. CONCLUSIONS: We have used SAM for the first time to provide nondestructive measurement of the in-vitro micro-mechanical properties of the soft and hard tissues that comprise the neonatal porcine TMJ. Because of the nondestructive nature of this technique the same specimens can be evaluated using complementary methods such as magnetic resonance microscopy thus, providing the opportunity to correlate micro-mechanical properties with micro-structural characterization and organization. Grant support from NIH/NIDCR T32 DE07294 and NIH S10 RR16710.
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| Seq #160 - TMJ Structure and Function 9:00 AM-10:30 AM, Friday, 11 March 2005 Baltimore Convention Center 331 | ||
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