| 2280 Cultured bone marrow cell combined with rhbFGF and rhBMP-7 | ||
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M. KATO1, J. SATO2, T. NAGANO3, K. GOMI1, T. ARAI1, and K. SETO1, 1Tsurumi University, Yokohama, Japan, 2Tsurumi University, yokohama, Japan, 3Tsurumi University, Kanagawa, Japan Objectives:Tissue engineering involving the in vitro growth and differentiation of harvested bone marrow cells (BMC) is considered a promising alternative to bone grafting for bone defect repair. In this study of growth factor substances, we cultured BMC in the presence of rhbFGF, which promotes cell growth, and rhBMP-7, which can induce cells to differentiate into osteoblasts and other cells. The carrier used was an atelocollagen (AC) solution. Methods:BMC were collected from Wistar rats, cultured in the presence of rhbFGF for 9 days (rhbFGF group), in the presence of rhbFGF for 6 days after 3 days of rhBMP-7 (rhBMP-7 group) and in the absence of the growth factor for 9 days (BMC group).Each group of BMC was recovered,suspended in AC solution carrier and subcutaneously injected into the back of nude mice. Injection with AC solution alone was used as the negative control (AC group).The mice were sacrificed 2 or 4 weeks later. The area (mm2) and volume (mm3) of bone-like tissue were measured by soft X-ray and micro-CT respectively, and then tissue sections were routinely processed for histopathological examination. Results:The mean areas and volumes of bone-like tissue and histopathologically, at 4 weeks, each group of BMC demonstrated increased bone-like tissue formation. The rhBMP-7 group especially demonstrated bone-like, bone marrow-like and fatty-like tissue. Conclusions:Thus, when an AC solution was used as a carrier, ectopic bone-like tissue was observed in the rhbFGF, rhBMP-7 and BMC groups. The rhbFGF group demonstrated less bone-like tissue formation than the BMC group because rhbFGF increased the number of BMC but could not induce the cells to differentiate into a significant amount of osteoblasts and other cells. In the rhBMP-7 group, the greatest bone-like tissue was formed because rhbFGF increased the number of BMC, and rhBMP-7 induced the cells to differentiate into osteoblasts and other cells. | ||
| Seq #248 - Reconstruction, Wound Healing 2:00 PM-4:00 PM, Friday, 11 March 2005 Baltimore Convention Center Exhibit Hall E-F | ||
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