| 1368 Insulin Mediates Bone Response in Diabetic Rats | ||
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M. MCCRACKEN1, R. CHAVALI1, R. APONTE-WESSON1, J. LEMONS2, and F. RAHEMTULLA1, 1University of Alabama, School of Dentistry, Birmingham, USA, 2University of Alabama, Birmingham, USA Objective: Diabetes continues to increase in prevalence and as an oral health concern. The purpose of this study was to investigate the bone response to implants placed in diabetic rat tibiae with and without insulin control. Methods: Institutional approvals were obtained for this project. This study reports data from 111 Sprague-Dawley rats randomly divided into three groups: Control, Diabetic, and Insulin. Diabetes was induced in the diabetic group with an injection of streptozotocin (65 mg/kg); the insulin group received streptozotocin (65 mg/kg) and a slow-release subdermal insulin implant; the controls received an injection of sterile water. Titanium alloy implants (1.5 x 8 mm screws) were placed in the proximal tibiae of the rats. Implants were allowed to heal for 2, 7, 14, or 25 days. Implants were retrieved and analyzed using histomorphometric techniques. Samples were processed in plastic and stained with toluidine blue. Percent bone tissue within 1 mm of the implant surface was quantified using imaging software (percent bone = percent bone pixels / percent area pixels). Data were analyzed using 2-way ANOVA (time and treatment effects) and Tukey's post-hoc analysis. Results: Overall, time and treatment effects were significant (p<0.0001). The mean proximal side bone percent values (±SE) were: Control, 13.7 (±1.6); Diabetic, 26.4 (±1.9); Insulin, 17.9 (±2.0). Post-hoc analysis revealed that diabetic bone response was significantly greater (p<0.01) than insulin and control groups, which were not different. A paired t-test demonstrated that proximal bone percent was significantly greater than distal bone percent (p<0.001). Mean percent bone response over time (±SE) was: 2-day, 8.4 (±1.4); 7-day, 27.3 (±1.8); 14-day, 24.0 (±1.8); and 24-day, 19.6 (±2.4). Conclusions: Diabetic animals produced significantly more bone adjacent to implants than controls. Bone response in insulin-treated diabetic animals was not significantly different from controls. Supported by 5R03DE014000-02. | ||
| Seq #156 - Growth Factors in Implantology Research in vivo 9:00 AM-10:30 AM, Friday, 11 March 2005 Baltimore Convention Center 329 | ||
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