| 2084 Tetracycline and Saline Washes Reduce Bacterial Contamination in Osseous Coagulum | ||
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A.W. ETCHESON1, D. MILEY1, and M.J. GILLESPIE2, 1Southern Illinois University School of Dental Medicine, Saint Louis University Graduate Periodontics, Alton, USA, 2Southern Illinois University, Alton, USA Studies have evaluated bone grafting materials and their regenerative potential. Autogenous bone is considered the “gold standard” with the greatest osteogenic potential for the treatment of periodontal defects. Objectives: This study assessed bacterial contamination of osseous coagulum obtained in quantities sufficient for grafting procedures and tested treatments for minimizing that contamination. Methods: Fifty osseous coagulum trap samples from patients undergoing implant osteotomy procedures were divided into groups of 10 and rinsed with normal saline, 0.12% chlorhexidine or 50 mg/ml tetracycline. Twenty control samples received no treatment. Samples were plated in triplicate on selective and differential media to determine the numbers of aerobic and anaerobic bacteria and to evaluate the presence of bacterial pathogens, especially staphylococci, streptococci, enterics, and black-pigmented bacteria (BPB). Plates were incubated at 37o C; anaerobic incubation was in a Coy chamber. Inoculations were performed with an Autoplate 4000. Results: Bacteria were isolated from all samples with total (aerobic plus anaerobic) colony forming units (cfu) ranging from 80 to 4 x 105/ml of suspended osseous coagulum. Viridans streptococci were isolated from 46 samples in numbers ranging from 40 to 1.3 x 105 cfu/ml. Enterics were in 16 samples with cfu ranging from 200 to 3.8 x 104/ml. Mannitol non-fermenting staphylococci were in one sample at 106 cfu/ml. BPB were not isolated. ANOVA with Scheffe post hoc tests indicated that, compared to control groups, there were significant reductions (p=0.05) in aerobic bacteria recovered from saline treated samples and total bacteria recovered from tetracycline treated samples. Conclusions: These data indicate significant bacterial contamination of osseous coagulum. The preponderance of viridans streptococci suggests the contaminants are primarily normal flora; however, pathogens were also observed justifying sample decontamination prior to bone grafting. Significant reduction in contamination was accomplished through saline and tetracycline treatments. Acknowledgement: Osseous Coagulum Traps were from 3i Inc. | ||
| Seq #231 - Clinical Research 2:00 PM-4:00 PM, Friday, 11 March 2005 Baltimore Convention Center Exhibit Hall E-F | ||
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