Enamel Matrix Derivative stimulates apoptosis of T cell-lymphocytes in vitro

*0222 Enamel Matrix Derivative stimulates apoptosis of T cell-lymphocytes in vitro*
B. SCHWENK¹, G. GASSMANN¹, S. LOVROV¹, A. SCULEAN², and W.-D. GRIMM¹, ¹University of Witten/Herdecke, Germany, ²University Nijmegen, Netherlands Introduction: Enamel Matrix Derivative (EMD) enhances periodontal regeneration. The mechanisms are not completely understood. Recently we showed Emdogain® to display significantly reduced biofilm vitality. In our 3D-collagen matrix migration model an influence on T lymphocyte migration has been revealed. Further FACS analysis showed dose dependent increase of CD25 activation and a dead cell fraction of CD4+ lymphocytes due to EMD. Thus it was the aim to reveal EMD dependent apoptosis of CD4+ lymphocytes. Material and methods: CD4+ lymphocytes were isolated immunomagnetically and incubated with EMD/RPMI (1, 25, 100µl/ml) in a 3-D collagen matrix migration model. After time-lapse video microscopy their migration was evaluated by computer assisted cell tracking. CD25 activation status and apoptosis was assessed by flow cytometry. Results: CD4+ cell migration was reduced in direct contact to EMD versus the controls (25µg: 21.8%±3.8, ctrl: 22.8%±6.5 p<0.023; 100µg: 24.1%±5.6, ctrl: 30.6%±6.5 p<0.001) but enhanced in indirect contact versus the controls (25µg: 26.1%±2.8, ctrl: 31.5%±5.2 p<0.001; 100µg: 42.9±4.8, ctrl: 47.2%±8.2 p<0.001). CD25 receptor activation was enhanced due to rising EMD concentrations in respect to the mean fluorescence intensity (25µg: 4.61mfi; 50µg: 4,82mfi, 100µg: 6.95mfi) versus the controls (3,61mfi= Iso-ctrl. and 4.41mfi= CD25-ctrl.). Apoptosis increased 3-6 fold dose dependently (1µg=13,70 MFI; 25µg=34,85 MFI; 100µg=64,07 MFI) versus the control (10,01 MFI). Discussion: Reduced biofilm formation (Arweiler et al. 2002) and dose dependent apoptosis of CD4+ lymphocytes may be regarded as a reason for uneventful healing due to EMD and thus to good soft tissue healing and to improved clinical attachment levels (Gassmann et al. 2003). Conclusions: EMD affects early states of biofilm maturation, activation of CD25 receptors of CD4+ lymphocytes and apoptosis and thus improves postoperative healing.
Seq #45 - Periodontal Regenerative Medicine/Tissue Engineering 9:00 AM-10:30 AM, Thursday, 10 March 2005 Baltimore Convention Center 338
Back to the Periodontal Research - Therapy Program Back to the IADR/AADR/CADR 83rd General Session (March 9-12, 2005)
Top Level Search