| 2279 Formation of a 3-D tissue engineering bone for mandibular repair | ||
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Y. HE1, Z. ZHANG2, J. LU2, X. JIANG2, Y. CAO2, and X. ZHANG2, 1Shanghai Second Medical University, Shanghai Ninth People's Hospital, China, 2Shanghai second medical university, DE, China Objectives: To construct an available tissue engineering bone combined bone marrow stromal cells(BMSCs) with 3-D scaffold Tricalcium Phosphate(TCP) in vitro in order to repair the mandibular segmental defect. Methods: After extract the dog's solution of marrow 2-3ml, the BMSCs were cultivated 각differentiated and expanded in vitro about 12 days and ultimately induced into the osteoblast cells with the systematic evaluations including the MTT각AKP and Von Kossa staining 각immunohistochemological examination of ON and OCN; To combined the active and enlarged bone marrow stromal cells (BMSCs) with the 3-D scaffold TCP which was shaped of the mandibular segmental defect designed and made by CAD/CAM and rapid prototyping technique. After the formation of the combined tissue in vitro about 5 day with the evaluations of TEM and SEM. The nude mouse implantation was used to explain the different situation of ossification in vivo. Results: After 12 days culture in vitro,the BMSCs was induced into the osteoblast cells in the numbers of 10-30 millions in third generated cells. The TEM displayed the normal ultra-structures including the nucleolus각mitochondria and endoplasmic reticulum and the immunhistochemical examination revealed the positive expression of ON and OCN .4 hours later, the SEM displayed the good attachment of the cells with materials, and after 5-7 days culture in the circumstance of the muti-holer material, the cells grows quite well and began to secret the matrix. The nude mouse implantation experiments revealed the group of cells with material had significant ossification situation compared with the group of simple material. 3 months later,we can see the mature osteoforming structure and lot of vascularized vessels under the HE staining. Conclusions: The BMSCs have the well inducing각differentiation and proliferation ability and could well be formatted into a tissue engineering bone with the mandible defect shaped scaffold TCP in vitro.
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| Seq #248 - Reconstruction, Wound Healing 2:00 PM-4:00 PM, Friday, 11 March 2005 Baltimore Convention Center Exhibit Hall E-F | ||
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