“Objectives:”, General: To compare the Bone Morphogenetic Protein-7 (BMP7) expression patterns in human dental pulp between healthy and injured teeth. Specific: To extract RNA from fresh human dental pulp of healthy and mechanically injured human teeth. To implement the real time RT-PCR for determining the BMP7 expression patterns.

“Methods:”, The samples were 24 carious free third molars, extracted for orthodontic reasons from 6 young adult patients. To determine the expression pattern of the BMP7, the injury was performed in vivo on 14 healthy teeth at different times before dental extraction: 0, 1, 24, 48 hours; 3, 5 and 15 days (each tooth injured at only one time). The control group consisted of 10 not injured teeth. Injury was realized on dentine by ClassI cavitary preparation, and covered with glass ionomer.

The pulps obtained immediately after dental extraction, were preserved in RNALater (Quiagen), frizzed in liquid Nitrogen and macerated to extract RNA with Trizol Reagent (Life Technologies). RNA was treated DNAse (Promega). Real Time RT-PCR was performed in DNAengine opticon (MJ Research) with Quantitec Sybrgreen (Quiagen) and specific primers for exons 1 and 2 of BMP7, designed by informatics alignment of BMP sequences. Control expression gene was human RNAr 18S. Statistical analysis was realized by Mean Comparison Test of threshold cycle (CT) and &Delta CT (CT BMP7 –CT ARNr18S) of injured teeth vs. not injured.

“Results:”, Transcriptional level of RNAr18S did not show statistically significant variations between injured and non injured pulps, but injured group exhibited a lesser BMP7 transcriptional level than the non injured (p<0.001).

“Conclusions:”, Real time PCR analysis suggests that mechanical injury in healthy teeth, induce a decrease in BMP7 pulp expression when compared to non injured. This pattern also was observed in each patient, independently of the time of injury.

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