Objectives: the aim of this study was to evaluate cytotoxicity effects of Zn-Al-Ag (Zinag)alloy and determine its suitable use as an alternative for gold Type I. Methods: In vitro studies were carried out on the Zn-Al-Ag samples using human fibroblast cells. Cytotoxicity of Zn-Al-Ag was checked by MTT test. This assay is based on the ability of mitochondrial dehydrogenases to oxidize thiazolyl blue (MTT), a tetrazolium salt (3-[4,5-dimethylthiazolyl-2,5-diphenyltetrazolium bromide), to insoluble blue formazan product. Cells were plated at 2.5 x 104 per well by triplicate and were incubated for 0, 3, 5 and 7 days. After each term, 50 ul of MTT were added and incubated for 3 hours. Then, the supernatant was removed, washed twice with PBS and 500 ul of DMSO was added to each well. Following 1 hr of slow shaking the absorbance was read at 570 nm. Results: Cytotoxicity test showed that Zinag (1%, 4%), had a positive response attaining values above the control,(polystyrene dish)in the day 0, however at day 3 the values decrease in both. In day 7 only the Zn-Al-Ag 4% showed a little activity. At day 7, only the control has shown activity. Conclusions: As Zinag alloy had a lower reaction in comparison with the control group new tests must be made to determine which of the components is presenting this cytotoxic effect.

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