Objectives: The aim of this study was to evaluate two used methods to apical microleakage test in root canals. Methods: Sixty maxillary canines, obtained from laboratory stock, were used. The crown-down technique was used, and the master apical file was a #50 K-file. The working length was established 1mm short of apex. During root canal instrumentation the teeth received 2.0 ml of distilled water to each instrument exchange. Finished this fase, teeth were randomly divided into 3 groups, one to each sealer: GI-Endofill, GII-Sealapex and GIII-Sealer 26. The root canals were obturated by lateral condensation technique. Root surface, except apical 2mm, was coated with two layers of cianoacrilate and each group was divided into 2 subgroups: SubGI- immersed in 2% methylene blue and SubGII- immersed in India ink. After 96 hours at 37ºC and 100% humidity, teeth of each subgroup were rinsed under tap water and submitted to two methods to visualize the level of apical microleakage: longitudinal fracture and decalcification and clarification process. Results: Data showed higher levels of apical microleakage in methylene blue groups (p<0.01) using both process, to Endofill and Sealer 26 sealers. When methylene blue dye was used, teeth sealed with Sealapex sealer showed lower (p<0.01) level of apical microleakage than the teeth that were immersed in India ink to both, longitudinal fracture and clarification process. Conclusion: It can be concluded that longitudinal fracture and clarification process enabled the evaluation of apical microleakage to both dyes, and that methylene blue dye promoted higher levels of apical microleakage than India ink, except to Sealapex sealer.

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