We have tried to induce the formation of cartilage tissue using water-soluble BMP which was prepared from crude BMP, and biodegradable membranes (Bio Mend and GC-MEMBRANE)and non-biodegradable membrane (GORE-TEX), these three kinds of membranes were used as scaffolds for the in vitro culture experiment. In previous in vitro culture experiment, we could induce of complete cartilage tissue only when GORE-TEX was used as a scaffold. The results were not stable. Objective: In this experiment, we tried to induce of cartilage tissue using crude BMP and the same membranes as scaffolds and also examined which one was the most effective. Methods: Crude BMP was extracted from bovine cortical bones according to Urist's procedure. Mesenchymal tissues from the forelimbs of fetal Sprague-Dawley rats at 20 days pregnancy were used. Crude BMP was dissolved in 1M urea, and dropped onto minced mesenchymal tissues. They were cultured for 2 weeks in a carbon dioxide incubator.Results: Complete cartilage tissue was observed only when GORE-TEXwas used as a scaffold. However, we observed cartilage-like tissue or calcified-like tissue formation when Bio Mend was used. We also observed partial cartilage tissue formation in case of using GC-MEMBRANE. These results were almost the same as the previous experiment, but the ratio of induction of cartilage tissue was increased. Conclusion: We confirmed again that GORE-TEX is the most effective material as a scaffold for this culture experiment.

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