| 0617 Examination of Clinical Efficacy of LTT to Metal Allergy Patients | ||
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K. NOKIBA1, M. MATUMURA1, K. WATANABE1, K. NAKAJIMA1, H. HIROHARA1, S. BABA1, M. KATO1, T. KOIZUMI1, T. MASUDA1, H. KITAZAKI1, H. HANI1, H. HAMANO1, T. OHYAMA2, and H. MIURA1, 1Tokyo Medical & Dental University, Graduate school Oral health Sciences, Japan, 2Tokyo Medical& Dental University, Japan Objectives: We investigated lymphocyte blast transformation test (LTT) for the patients with a suspected metal allergy attended the outpatient allergy clinic of the Dental Hospital at TMDU. The aim of this study was to evaluate the clinical efficacy of LTT using metal reagent and peripheral blood of patients by comparing conventional patch test(PT). Methods: We focused on Ni, Pd and Au. Ni is high PT positive metal and we often use Pd and Au alloys in dental treatment. Peripheral blood mononuclear cells (PMBC) were separated by centrifugation over Conray-Ficoll. PMBC in RPMI1640 complemented with homologous plasma were triplicated at 2x105 cells per well in flat-bottom 96well plates without or with NiSO4, PdCl2 and HAuCl4. After 3 days, plates were supplemented with [3H]thymidine. Cells were cultured for 16 hours and the radioactivity was measured. A positive lymphocyte response was defined as stimulation index over 181%. Results: The result of LTT correspond with PT were represented by sensitivity and specificity. The sensitivity was Ni (100%), Pd(42.9%), and Au(25%), and the specificity was Pd(100%), Au(81.8%), and Ni(0%) as the same patients according to each metal. Moreover,The results of LTT have some tendencies patient's skin lesion. It was suggested that responses on Ni was non-specific blast transformation. Conclusions: LTT operated carefully choosing metal elements and cases can supplement PT results. | ||
| Seq #80 - Amalgam and Biocompatibility 10:15 AM-11:30 AM, Thursday, 11 March 2004 Hawaii Convention Center Exhibit Hall 1-2 | ||
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Back to the Dental Materials: VII - Others-Metallic Program
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