Objectives: The purpose of the present study was to evaluate the sealing ability of a newly developed sealing material and various commercialized water-based temporary sealing materials in vivo. Methods: The materials used in the present study were Caviton, HY-seal, Lumicon, Cavit-G, Cavit-W and CT-7, the last being the newly developed one. Standard access cavities were prepared in 120 intact extracted human molar teeth. The teeth were randomly divided into 6 groups of 20 teeth each and sealed with the test materials, respectively. Fifteen teeth each of the resulted groups were immersed in 1% methylene blue solution at 37 degree C for 7, 14 or 28 days, and the rest 5 teeth each were submitted to thermal cycling test (4 degree C: 1 minute, 60 degree C: 1 minute) for 30 cycles in 1% methylene blue solution. After immersion for 7, 14, and 28 days or thermocycling, the teeth were dried at 37degree C for 24 hours. They were split in half (buccal- lingual), and the depth of dye penetration into the margins of the tooth surface were measured with a digital micrometer caliper. The results were analyzed by Student's T test. Results: The results indicated that the depth of dye penetration with Lumicon, Caviton, and CT-7 was up to 2.5 mm and over 5.0 mm with HY-seal and Cavit-G at 28 days. The difference between the former and latter was significant (p<0.01). The depth of dye penetration with CT-7 in the thermal cycling test for 30 cycles was similar to that with Caviton and HY-seal and shallower than that with Cavit-G and Cavit-W (p<0.01). Conclusion: Our results suggest that the newly developed CT-7 may possibly be used clinically as a water-based temporary sealing material.

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