“Objectives:” This research analyses the sealing capacity and the marginal adaptation in vitro of a few materials when utilized under furcal perforations. “Methods:” A hundred and forty four extracted human molars. From the total amount, eighty-eight were utilized for checking the sealing and sixteen for checking the marginal adaptation under S.E.M. Following that, there was an impermeabilization of the whole external surface of the specimens, and the inclusion in a heavy silicon base. There was a 1.4mm perforation to the center of the floor of each specimen. In each group, subgroups were created. One receiving a Plaster of Paris matrix and the other no. The obturation of perforations were then, made with the following materials: GI (MTA–Angelus), GII (ProRoot–MTA), GIII (Super-EBA), GIV (MBP-c-experimental). After that, the specimens were submerged in a rhodamine B solution (37°C-48hs). The reading of the marginal adaptation was performed by an optical microscope utilizing numerical scores. For the S.E.M analysis the specimens were submitted to the same methodology except for the steps of impermeabilization and the submerging in dye, then photos with 50X e 200X were obtained. “Results:” The statistical analysis revealed that when isolated and tested (without the matrix), the cement MBP-c presented the smallest coefficient of marginal leakage and marginal adaptation, followed by the Super-EBA. There was a statistical significance between those and the cements ProRoot – MTA e MTA – Angelus (p<0,001). In the presence of the matrix, the cement MBP-c also had a superior performance having statistical significance between those and the cements ProRoot – MTA e MTA – Angelus (p<0,05); “Conclusion:” The Plaster of Paris matrix had a negative impact with statistical significance regarding the sealing capacity of the cements Super-EBA and the MBP-c (p<0,05).

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