Objectives: We investigated the effect of Mineral Trioxide Aggregate(MTA) on human periodontal ligament fibroblasts(HPLF) stimulated with Lipopolysaccarides in vitro by measuring gene expression of the inflammatory cytokines IL-1a,b,IL-6,IL-8 and TNF-a. Methods:We cultured HPLF which had been isolated from the periodontal ligament of freshly extracted human teeth. MTA used in the experiment was mixed according to the manufacturer's instruction. Cells were grown to confluence and divided into four groups. Group1 had no stimulation(control), Group2 was stimulated with LPS(10µg/ml), Group3 was stimulated with MTA, and Group4 was stimulated with both LPS(10µg/ml) and MTA. After 3,6,12 and 24 hours of culturing, total RNA was extracted and a real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was performed to measure the expression of mRNA. All data were evaluated by ANOVA and Turkey. Results:We found that IL-1a and b gene expression in Groups2, 3 and 4 was similar to that in Group1. IL-6 and IL-8 gene expression after 3 hours of culture was greater in Group4 than in the other three groups. The expression gradually decreased up to 24 hours of culture, when it became similar to that of the control . TNF-a gene expression in Groups2 and 4 was greater than in Groups1 and 3. TNF-a gene expression was similar in Groups1 and 3. Conclusions:The results of this study showed that MTA was ineffective at controlling inflammation on HPLF stimulated with LPS.

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