Objectives: The mechanisms of the occurrence and evolution of squamous carcinoma haven't been clarified yet. In the past, many studies only focused on the changes of epithelium or mesenchyme but neglecting the important interactions between the epithelium and mesenchyme. This subject intended to apply the new theory of microecology of the tumor-host interface to the field of the oral tumor. The major adjustment factor as carcinoma-associated fibroblasts (CAFs) was selected as the research topic. However, no related papers about oral CAFs were reported. So the purpose of this study was to ¢ŁTo separate and culture and verify the oral CAFs preliminarily; ¢ŚTo study the main biologic characteristics of the oral CAFs. Methods: ¢ŁThe primary CAFs and NFs were obtained by tissue culture, cells were passaged by 0.25% trypsin and purified by curage method and trypsinization; ¢ŚTo verify the cells preliminarily according to the changes of morphology and the expression of the certain proteins; ¢ŪTo compare the CAFs with NFs of their biologic characteristics, such as proliferation and motility and karyotype etc. Results: ¢ŁThe oral CAFs were successfully separated and cultured and verified preliminarily; ¢ŚCompare to the normal fibrolasts from oral mucosa, the proliferation and motility of the oral CAFs increased significantly. However, the basic characteristics of the normal cells were kept in the oral CAFs, which meant the oral CAFs had no malignant changes. Conclusions: ¢ŁThere are obvious differences of the biologic characteristics between the CAFs and NFs; ¢ŚThe microecology of the oral tumor-host interface might be one of the most important factors affecting the biologic characteristics of the CAFs.

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