| 1050 Relationship between Oral Malodor and Clinical States of Periodontal Disease | ||
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T. ARAI1, A. HAYASHIDA2, K. HOSHI2, A. NAWASHIRO3, M. KISHI4, S. IMAI5, N. HANADA5, and K. KAMOI1, 1Nippon Dental University, Tokyo, Japan, 2Tokyo Medical and Dental University, Japan, 3Nippon Dental University Hospital, Tokyo, Japan, 4Iwate Medical University, Japan, 5National Institute of Public Health, Tokyo, Japan Objectives: Volatile sulfur compounds (VSC) are produced by various periodontopathic microorganisms, and Porphyromonas gingivalis has been reported to be one of the highest active VSC producers in vitro. The purposes of this study were to measure odor compounds from culture of P. gingivalis periodically and to investigate the relationship among oral malodor, clinical score, number of periodontopathic microorganisms in saliva, tongue coat and gingival crevicular fluid (GCF). Methods: P. gingivalis ATCC33277 was grown in 4ml of modified BM medium containing hemin and menadione supplemented with 30µ of 33mM L-cysteine or L-methionine in an anaerobic chamber ( N2, 80%; H2, 10%; CO2, 10%) at 37oC. The tube was picked out one by one periodically, and the gas of head space was measured by simplified Gas Chromatograph ("OralChroma¨", ABILIT, Japan) and the Electronic Nose (Fragrance and analyzer "FF-1¨", SHIMADZU, Japan). Twenty subjects (7 males and 13 females; mean age 48.95) who visited the General Dentistry, Nippon Dental University Hospital were recruited. The clinical examination comprised probing pocket depth (PPD), gingival index (GI), bleeding on probing (BOP), organoleptic score (OLS), clinical attachment level (CAL) and coated tongue score. The score of VSC were analyzed by absolute odor expression software ("ASMELL¨", SHIMADZU, Japan). The statistical analysis was performed by Student's t test or Spearman's rank-order coefficient test. Results: The pattern of growth curve in P. gingivalis was consistent with the pattern of VSC production. Average GI, BOP and PPD in MMC detected group (N=10) were significantly higher than that in MMC non-detected group (N=8)(p<0.05). MMC/H2S ratio correlates significantly with OLS (r=0.509, p<0.05), and with average GI (r=0.531, p<0.05). There were also significant positive correlations between average PPD and quantity of P. gingivalis in tongue coat (r=0.88, p<0.021). Conclusions: Our data suggest that states of oral malodor will correlate with clinical states of periodontal disease. | ||
| Seq #112 - Diagnostics: Local and Systemic Factors I 10:15 AM-11:30 AM, Thursday, 11 March 2004 Hawaii Convention Center Exhibit Hall 1-2 | ||
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