| 3514 Bacterial Adhesion on Integrated Abutment CrownsTM. In Vivo Study (II) | ||
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M. MARINCOLA1, S. DIBART2, M.L. WARBINGTON3, Z. SKOBE3, R. URDANETA4, and S.-K. CHUANG5, 1University of Cartagena, AISI, Italian Association of Restorative Implant Dentistry, Rome, Italy, 2Boston University, MA, USA, 3Forsyth Institute, Boston, MA, USA, 4Harvard University, Concord Dental Associates, Concord, MA, USA, 5Massachusetts General Hospital and Harvard School of Dental Medicine, Harvard School of Public Health, Chestnut Hill, USA Objectives: The purpose of this study was to compare the subgingival microbiota present on implant supported Integrated Abutment Crowns (IAC) and natural teeth in vivo. Material and Methods: A cross-sectional study design was utilized with patients selected from the patient pool at the implant dentistry center at Faulkner Hospital (Boston, MA). Thirty-one patients (13 males and 18 females) were selected, mean age 57.36 years (range 28.09 to 90.85 years) of which 4 were smokers. Selection requirements were: Patients had IAC crowns placed at least 6 months ago and had not taken antibiotics 3 months prior evaluation. Gingival index (GI), modified bleeding index (MBI), subgingival plaque samples and clinical photographs were taken on at least 1 IAC and the natural contralateral tooth on each patient. The subgingival plaque samples were taken from the mesial side of the IAC or natural teeth and put in an Eppendorf tube containing 0.150 ml Tris-EDTA. The samples were then hybridized with 12 whole chromosomal probes to Tannerella forsythensis, Prevotella intermedia, Campylobacter rectus, Fusobacterium nucleatum, Actinomyes odontolyticus, Streptococcus sanguis, Streptococcus intermedius, Actinobacillus actinomycetemcomitans serotype b, Actinomyces naeslundii, Streptococcus oralis, Porphyromonas gingivalis and Prevotella intermedia , using the checkerboard DNA-DNA hybridization method. The descriptive statistics and generalized linear mixed models (GLMM) accounted for intra-cluster correlation within the same patient were utilized using SAS-PC (version 8.2, 2001) Results: IAC were noted to have less GI and MBI compared with natural teeth but were not statistically significant (p>0.05). There were no statistical differences (p>0.05) in all the various colonies count between IAC and the natural teeth. Conclusions: The IAC showed striking similarities with the natural tooth in terms of subgingival bacteria plaque count and composition. The IAC also showed lower GI and MBI indices. Supported by a research grant from Bicon, Inc.(MM, SD), OMSF Foundation Fellowship in Clinical Investigation (SKC) | ||
| Seq #360 - Biomechanics/Implant Surfaces 10:15 AM-11:30 AM, Saturday, 13 March 2004 Hawaii Convention Center Exhibit Hall 1-2 | ||
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