Bacterial Adhesion on Integrated Abutment CrownsTM. In Vitro Study(I)

*3515 Bacterial Adhesion on Integrated Abutment CrownsTM. In Vitro Study(I)*
S. DIBART¹, M. MARINCOLA², M.L. WARBINGTON¹, and Z. SKOBE³, ¹Boston University, MA, USA, ²University of Cartagena, AISI, Italian Association of Restorative Implant Dentistry, Rome, Italy, ³Forsyth Institute, Boston, MA, USA Objectives: The goal of the present investigation was to determine if the Diamond-crown material, used to make Bicon's Integrated Abutment Crown (IAC),is less susceptible to harbor/attract bacterial plaque than All Ceramic (AC) or Metal Ceramic (MC) crowns. A secondary goal was to compare the composition of the plaque attracted on tested surfaces. Methods: 6 IAC, 6 AC and 6 MC crowns were equally divided in 2 test groups. The AC and MC crowns were cemented on titanium abutments. Group I (3 IAC,3 AC, 3 MC)and group II were incubated for 10 min in a bacterial solution containing 10 different oral bacteria at O.D.1: Tanerella forsythensis, Prevotella intermedia, Campylobacter rectus, Fusobacterium nucleatum, Actinomyces odontololyticus, Streptococcus intermedius, Actinobacillus actinomycetemcomitans serotype b, Actinomyces naeslundii, Streptococcus oralis, Porphyromonas gingivalis. After a brief wash in PBS to eliminate the unbound plaque, the crown samples in group I were incubated in 500痞 of Tris-EDTA buffer with 500痞 of NaOH. The samples were then hybridized with 10 whole chromosomal probes to the above mentioned microorganisms. The microbiological analysis was completed using the checkerboard DNA-DNA hybridization method. The samples in group II were briefly washed in PBS and fixed in 4% formalin for scanning electron microscopy (SEM) Results: All experimental crowns showed bacterial adhesion. There was no statistical difference in the microbial compositions when comparing crowns. The SEM showed that the AC crowns were harboring the heaviest bacterial deposits. MC and IAC showed the least bacterial deposits especially at the abutment/crown interface. Conclusions: The IAC and MC crowns appear to be less prone to bacterial colonization, in an in vitro setting then an all ceramic crown. IAC and MC crowns harbored very few bacteria at the abutment/crown interface This study was supported by a research grant from Bicon,Inc.
Seq #360 - Biomechanics/Implant Surfaces 10:15 AM-11:30 AM, Saturday, 13 March 2004 Hawaii Convention Center Exhibit Hall 1-2
Back to the Implantology Research Program Back to the IADR/AADR/CADR 82nd General Session (March 10-13, 2004)
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