| 1047 Detection of Cystatins in Saliva Using Murine Monoclonal Antibody | ||
|
T. ITO1, A. KOMIYA1, S. YAMADA1, K. MINAGUCHI2, E. SAITOH3, K. OKUDA1, and T. KATO1, 1Tokyo Dental College, Chiba, Japan, 2Tokyo Dental College, Chiba City, Japan, 3Nippon Dental University, Niigata, Japan Objective: Cystatins in saliva have been studied as possible protective proteins against the process of tissue destruction. Therefore, measuring the concentrations of salivary cystatins in whole saliva may provide a useful tool for monitoring periodontal disease status. We tried to detect the family 2 cystatins in saliva samples using a monoclonal antibody. Methods: In order to detect the family 2 cystatins in saliva, we produced monoclonal antibodies against cystatin SA. We tried to detect the cystatin levels in saliva samples by dot blot assay using monoclonal antibody Cys3F11, which reacts with both cystatins SA1 and SA2. In the clinical application study, the protocol was reviewed and approved by the Review Board of Tokyo Dental College. 20 patients who visited Tokyo Dental College for periodontal treatment were chosen as subjects in the periodontally diseased group. Twenty medical personnel of Tokyo Dental College with clinically healthy periodontia were employed as the healthy group. The detection levels of family 2 cystatins in the saliva samples were carried out on the basis of using the reaction signals of recombinant cystatin SA1 as the standard. Results: We succeeded in detecting the family 2 cystatin levels in our saliva samples using the Cys3F11 monoclonal antibody. The cystatin levels in saliva samples from the periodontally diseased group had a mean value of 0.08±0.03 mg/ml, which was statistically signigicantly lower than that of the healthy group ( p<0.05 ). Conclusion: We showed that the cystatin levels of the healthy group were higher than those of the periodontally diseased group. The present study suggests that our dot-blot method using this monoclonal antibody is a useful tool in measuring cystatin levels in whole saliva to monitor periodontal disease status. This study was supported by Oral Health Science Center Grant 5A04 from Tokyo Dental College. | ||
| Seq #112 - Diagnostics: Local and Systemic Factors I 10:15 AM-11:30 AM, Thursday, 11 March 2004 Hawaii Convention Center Exhibit Hall 1-2 | ||
|
Back to the Periodontal Research - Diagnosis / Epidemiology Program
| ||