Elutable substance and degradation products from root end sealer may affect the surround tissue. Objective: The aim of the present study was to comparison various root end sealers cytotoxicity and genotoxicity. Materials and Method: Three root end sealers, Super EBA, Life and MTA were used. Single cell suspensions of human osteosarcoma cell line (U2OS) were seeded in complete McCoy medium. After the exposure to root end sealers, cell viability was determined by the ability of the cells to cleave the tetrazolium salt (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) (MTT) to a formazan dye. The DNA strand break assay was used to investigate the pathobiologic effects. The results were compared using the one-way analysis of variance (ANOVA). Differences of the treatment means were analyzed by the Student-Newman-Keul's test and were considered to be significant at p<0.05. Results: The survival rate of the MTA showed no statistical difference as concentration increased (p>0.05). The survival rate of Super EBA and Life showed dose dependent decrease as concentration increase (p<0.05). The Super EBA and Life root end sealers showed DNA ladder on electrophoretic assay, but not the MTA. Conclusion: The MTA root end sealer showed biocompatible with U2OS cells. The Super EBA and Life showed cytotoxicity and genotoxicity on U2OS cells. This research was supported by Taiwan National Scientific Center (NSC 91-2314-B040-007).

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