| 3829 Measurement of oxidative stress in rat TMJ arthritis model | ||
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Y. KAWAI1, H. SHOJI2, H. MIYAZAKI3, M. SUGA4, S. SHIMIZU4, H. KATO1, M.C.-I. LEE5, and E. KUBOTA4, 1Kanagawa Dental College, Department of Oral & Maxillofacial Surgery, yokosuka, Japan, 2Kanagawa Dental College, Department of Pharmacology, yokosuka, Japan, 3kanagawa dental college, Department of Oral & Maxillofacial Surgery, yokosuka, Japan, 4Kanagawa Dental College, Department of Oral & Maxillofacial Surgery, Yokosuka, Japan, 5Kanagawa Dental College, Department of Pharmacology, Yokosuka, Japan Objectives: Oxidative stress due to the generation of reactive oxygen species (ROS) has been implicated in the pathogenesis of a variety of inflammatory diseases. Using the electron spin resonance (ESR)/spin trap technique, we have previously demonstrated that hydroxyl radical (HO¥) could be generated by catalytic reaction of H2O2 with free iron ions (Fenton reaction) in IL-1a-induced rat temporomandiblar joint (TMJ) arthritis model. In vivo ESR technique, including ESR imaging can provide a useful tool for non-invasive assessment of oxidative stress in the rodent model. In the present study, we evaluated the oxidative stress of the TMJ region by using in vivo L-band ESR. Methods: TMJ arthritis was experimentally induced in rats by injection with the rat recombinant IL-1b or TNF-a into the TMJ. Control rats were treated with normal saline solution. We used a 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-1-yloxy (C-PROXYL) as a nitrooxide spin probe, and performed the measurement of signal decay rate of C-PROXYL in the TMJ region by using in vivo L-band ESR. Results: The decay rate of C-PROXYL non-invasively measured by L-band ESR in IL-1b-induced arthritic TMJ was much higher than that in the control TMJ at 12 h and 48 h after the IL-1b injection. The decay rate of C-PROXYL in TNF-a-induced arthritic TMJ was much faster than that in the control TMJ at 48 h and 1 week after the TNF-a injection. We also succeeded in reconstructing 3D images of the distribution of C-PROXYL in the TMJ region of the live animal non-invasively. Conclusion: We could measure the oxidative stress in the IL-1b- or TNF-a-induced rat TMJ arthritis model by L-band ESR. With further advancement in in vivo ESR technique, this technology holds great promise for non-invasive assessment of oxidative stress in the TMD. | ||
| Seq #385 - TMJ Structure and Function 10:15 AM-11:30 AM, Saturday, 13 March 2004 Hawaii Convention Center Exhibit Hall 1-2 | ||
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