| 1090 Defining Appalachian Communities by Antibiotic Resistance Profiles for Oral Staphylococcus aureus | ||
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J.G. THOMAS1, D.E. YAKUBU2, J. KARAKIOZIS1, C.A. MARTIN1, R.J. CROUT1, W. BRETZ3, and R. WEYANT3, 1 West Virginia University, Morgantown, USA, 2 Aberdeen University Medical School, United Kingdom, 3 University of Pittsburgh, PA, USA West Virginia population exhibits severe dental disease with 1/3 of the adult population under 35 missing 6 or more teeth, while 31% are edentulous. A pilot study was performed in 2000 for two Appalachian counties (Webster and Nicholas) of WV to obtain health behavioral information, oral and pharyngeal microbial specimens from sentinel families. The initial culture comparisons for Staphylococcus aureus , Candida albicans and Streptococcus pyogenes were reported earlier. OBJECTIVES: Here, we report : 1) MICs , 2) Resistant phenotypic patterns, 3) MIC 50 /MIC 90, and 4) cluster of similar resistance clone for six oral antibiotics to pharyngeal Staphylococcus aureus, comparing the data to South East Regional, US data and WVU Hospitals. METHODS: Susceptibility testing was performed on pharyngeal Staphylococcus aureus isolates from 56 mother- child pairs including 21 mother-father-child participants by the Etest, a gradient-elution technique (AB Biodisk, Upsala, Swenden). A dendrogram for resistant cluster analysis was compiled from MIC data using the method of D.E. Yakubu. Electronic surveillance for comparative resistant profiles was compiled via the TSN Database - USA (Herndon, VA). RESULTS: Distinct differences (p<- 0.05) among regions were recognizable with the Resistant Phenotypes, the MIC Distribution and the comparison of MIC 50/MIC 90 for 28 positive pharyngeal Staphylococcus aureus specimens. Clindamycin showed the greatest variability. Isolates from the Appalachian Counties had lower MIC's and were more homogeneous. Three most resistant antibiotics were cipro>oxacillin> erythromycin. Resistant cluster analysis of the Appalachian isolates revealed seven multi-isolate resistant clusters, with two to seven Staphylococcus aureus members in each, the largest comprising one quarter of all the isolates tested. Using Dice Coefficient of Similarity of 70-80% suggested that the child isolates were derived from those of their parents. CONCLUSION: Staphylococcus aureus MIC data analysis by 4 methods revealed significant differences for resistant profiles from the US, Southeast and WVUH compared to two Appalachian WV counties. This study is the first to use oral MIC data to unmask isolate relatedness and define similarities amongst a closed population. | ||
| Seq #139 - Diagnosis/Epidemiology 11:00 AM-12:15 PM, Friday, 14 March 2003 Henry B. Gonzalez Convention Center Exhibit Hall C | ||
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