Longitudinal Monitoring of Microbiota of Periodontal Health

*1088 Longitudinal Monitoring of Microbiota of Periodontal Health*
S.C. LU¹, R.L. KENT¹, L.A. MURRAY¹, A.J. ORTEGA², S.T. SONIS³, T.E. VAN DYKE⁴, and A.C.R. TANNER¹, ¹ The Forsyth Institute, Boston, MA, USA, ² Harvard School of Dental Medicine, Boston, MA, USA, ³ Brigham and Women's Hospital/Harvard School of Dental Medicine, Boston, MA, USA, ⁴ Boston University Goldman School of Dental Medicine, MA, USA Objectives: This study sought to evaluate whether changes in the subgingival microbiota could be detected during longitudinal monitoring of periodontally healthy subjects. Methods: 66 medically and periodontally healthy subjects (=all mean periodontal AL £1.8 mm), 20-40 yr, were measured using full mouth (168 sites) measures of pocket depth (PD), attachment level (AL), gingival index (GI), plaque index (PI), and % sites bleeding on probing (BOP) at 6-month intervals for 18 mos. Subgingival plaque samples from 8 mesial molar sites were assayed using a whole genomic DNA probe checkerboard assay to 10 species. Subjects received supragingival polishing at each visit, but not subgingival scaling. Associations of the microbiota with clinical features, and changes in the microbiota during monitoring were sought. Results: The 36 female and 30 male subjects had meanąsd values for: age 29.5 ą 6 yr, AL 1.28ą0.23 mm, PD 2.05ą0.20 mm, GI 0.90ą0.52, PI 0.92ą0.59. 75% subjects were never smokers, 8% Asian, 12% African American, 5% Hispanic, and 71% Caucasian. By site, proportions of S. intermedius, A. actinomycetemcomitans, C. gingivalis, E. corrodens, C. rectus, K. oralis, P. endodontalis, P. gingivalis, and T. forsythensis changed little over time. Proportions of A. odontolyticus were higher at the end than the beginning of monitoring. Higher proportions of T. forsythensis, and P. gingivalis were detected associated with BOP sites and increased PD. A. odontolyticus was detected in higher proportions in shallow, non-BOP sites. Conclusions: Periodontal species were detected in periodontally healthy subjects over 18 mos monitoring suggesting generally stable colonization. The association of periodontal pathogens with increased site pocket depth and bleeding on probing in healthy subjects suggests that early and later phases of periodontitis have a similar etiology. Supported by NIDCR Grant DE-09513.
Seq #139 - Diagnosis/Epidemiology 11:00 AM-12:15 PM, Friday, 14 March 2003 Henry B. Gonzalez Convention Center Exhibit Hall C
Back to the Periodontal Research - Diagnosis / Epidemiology Program Back to the 32nd Annual Meeting and Exhibition of the AADR (March 12-15, 2003)
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