| 0595 Morphological FE-SEM Observations of Hybrid Layer after Ar-ion Etching | ||
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N. AKIMOTO1, G. YOKOYAMA1, and C.F. COX2, 1 Tsurumi University, School of Dental Medicine, Yokohama, Japan, 2 UCLA School of Dentistry, Los Angeles, CA, USA Objective: Inokoshi et al. 1990 employed Argon ion beam etching (Ar-ion etch) with SEM observation to the hybrid layer of the resin-dentin interface, describing the hybrid layer as composed of a meshwork structure. It is not clear if this meshwork configuration is resin or collagen fiber. The purpose of this study was to examine the hybrid layer after Ar-ion etching by FE-SEM (ERA-8800FE, Elionix, Tokyo, Japan). Methods: Over 6 dentin discs were prepared from freshly extracted human third molars, the surfaces intentionally over-etched for 60-sec's, with 32% H3PO4 (Uni-etch, Bisco, Schaumburg, USA) rinsed and gently air dispersed. These surfaces were treated with Clearfil SE Bond (Kuraray Medical, Tokyo, Japan), and Superbond C&B (Sun Medical, Kyoto, Japan) respectively following manufacturer's directions. Specimens were restored with a flowable resin (Revolution, Kerr/Sybron, Orange Co., USA), stored in water for 1-day, longitudinally sectioned through the resin-dentin interface and the surface polished with diamond paste. Each specimen was submitted to three sequential treatment procedures. Each specimen was Ar-ion etched for 30-sec's with 1 kV acceleration voltage (EIS-200ER, Elionix, Tokyo, Japan), and the resin-dentin interface observed by FE-SEM. The same area was again Ar-ion etched for 10-secs, treated with 10% NaOCl for 1-min and observed by FE-SEM. Lastly, the same area was Ar-ion etched and treated with 10-3 solution (Sun Medical) for 1-sec. Results: Ar-ion etching alone demonstrated a hybrid layer meshwork configuration similar to that previously reported. Ar-ion etching, NaOCl and 10-3 treatment revealed no change to the structure of the hybrid layer. However, following NaOCl treatment, FE-SEM evaluation demonstrated the meshwork configuration had only partially disappeared between the hybrid layer and dentin substrate. Conclusions: As the meshwork is similar following various treatments, our data suggests that the unique hybrid layer meshwork configuration is composed of both polymerized resin and partially exposed collagen fibers. | ||
| Seq #82 - Adhesive Interface Microstructure 3:45 PM-5:00 PM, Thursday, 13 March 2003 Henry B. Gonzalez Convention Center Exhibit Hall C | ||
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