| 1517 Post-operative Cytokine Therapy Allows for Normal Intracranial Volume in Craniosynostotic Rabbits | ||
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M.P. MOONEY1, H.W. LOSKEN2, A.M. MOURSI3, R. MITCHELL1, W. WIGGINTON1, P.L. WINNARD3, J. BRADLEY1, K. AZARI1, O. ACARTURK1, L.A. OPPERMAN4, E. STELNICKI5, and M. SIEGEL1, 1 University of Pittsburgh, PA, USA, 2 University of North Carolina, Chapel Hill, USA, 3 Ohio State University, Columbus, USA, 4 Baylor College of Dentistry, USA, 5 Cleveland Clinic, Miami, FL, USA Objectives: A high expression of Transforming growth factor-b2 (Tgf-b2) is related to premature suture fusion, postoperative resynostosis, and secondary decreases in intracranial volume (ICV). Interference with Tgf-b2 function may prevent such secondary anomalies. The present study was designed to test this hypothesis in a craniosynostotic model. Methods: 107, 3D-CT scans were collected from 46 New Zealand white rabbits. The rabbits were divided into 5 groups: 1) Normal controls (n=14); 2) Unoperated synostosed controls (n=12); 3) Synostosed with suturectomy only (n=7); 4) Synostosed with suturectomy and control IgG antibody (n=5); and 5) Synostosed with suturectomy and anti-Tgf-b 2 antibody (n=8). At 10 days of age, a 3mm x 10mm coronal suturectomy was performed in rabbits in Groups 3-5. The sites in Groups 4 and 5 were immediately filled with 0.1cc of a slow resorbing (90-180 day), collagen gel mixed with either a non specific IgG antibody (100mg/suture) or anti-Tgf-b2 antibody (100mg/suture). 3D-CT scan reconstructions of the defects were obtained at 10, 25, 42, and 84 days of age. The heads were scanned at 1mm sagittal cuts and the endocortical boundaries of the cavities were traced, reconstructed, and IC volumes calculated. Results: Two-way analysis of variance revealed significant age (F=326.1; p<0.001) and group (F=4.3;p<0.001) effects. Rabbits that were treated with anti-Tgf-b2 antibody showed significantly (p<0.05) greater ICVs at all ages compared to unoperated, suturectomy only, and IgG control rabbits and were not significantly (p>0.05) different from normal control rabbits by 42 days of age. Conclusions: These data support our initial hypothesis that an interference with Tgf-b2 can ameliorate secondary ICV reductions. These findings also suggest that this cytokine therapy may have clinical potential in infants with craniosynostosis. Supported by NIDCR (DE13078). Collagen provided by NeuColl Inc., Campbell, CA. | ||
| Seq #191 - Craniofacial Development 9:00 AM-11:00 AM, Saturday, 15 March 2003 Henry B. Gonzalez Convention Center Room 213B | ||
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